Determination biochemical differences and rapid ıdentification of yersinia ruckeri and lactococcus garvieae ısolated from aquaculture

Abstract

Aquaculture has growing much more than 100% at the last decade. Rainbow trout is the most economically important fish among the aquatic species. Bacterial agent, especially, Yersinia ruckeri and Lactococcus garvieae have limited freshwater fish production all over the world. There are many reports about identification of bacterial pathogens in aquaculture but most of them should not supported with detailed biochemical features. Therefore, we aimed in this study, determination of biochemical differences and identify two bacteria in a short time by using rapid test kits. 30 isolates of each Y. ruckeri and L. garvieae that are represent different regions of Turkey were used and biochemical characteristics were determined by conventional microbiologic test and API RAPID test kits. Y. ruckeri is the more heterogenetic biochemical structure comparing to L. garvieae. All isolates were successfully identified within 5-7 hours with rapid test kits while there are some differences between each other. The comprehensive biochemical differences of Y. ruckeri and L. garvieae were shown in the present study