Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/17369
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dc.contributor.authorUğur, Aysel-
dc.contributor.authorCeylan, Özgür-
dc.contributor.authorAslım, Belma-
dc.date.accessioned2021-03-10T08:21:14Z-
dc.date.available2021-03-10T08:21:14Z-
dc.date.issued2012-
dc.identifier.citationUğur, A. vd. (2012). "Characterization of Pseudomonas spp. from seawater of the southwest coast of Turkey". Journal of Biological and Environmental Sciences, 6(16), 15-23.tr_TR
dc.identifier.issn1307-9530-
dc.identifier.issn1308-2019-
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/497525-
dc.identifier.urihttp://hdl.handle.net/11452/17369-
dc.description.abstractIn this work, 46 gram negative bacteria isolated from the seawater on the southwest coast of Turkey. These isolates were identified as 20 Pseudomonas spp., 15 Aeromonas spp., 8 Chryseomonas spp., 2 Burkholderia spp. and 1 Vibrio spp. by biochemical and conventional methods. And then study was continued for the 20 Pseudomonas isolates. Species identification of these isolates were made by means of API 20 NE. Enzymatic activities of these strains were determined by API ZYM. The strains were further characterized by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and 16S ribosomal DNA sequencing. According to the results obtained by phenotypic methods; the 20 isolates were identified as 6 Pseudomonas aeruginosa, 7 P.fluorescens, 3 P.putida, 2 P.stutzeri, 1 P.mendocina and 1 P.alcaligenes. The results of protein profiles and 16S rDNA sequence correlated well with phenotypic classification for P.aeruginosa, P.putida, P.stutzeri and P.mendocina strains. Good overall agreement between phenotypic and genotypic identification procedures was found for the 17 Pseudomonas spp. But discordance of identification between phenotypic methods and 16S rDNA sequence analysis was observed for two P. fluorescens (P 5-4 and P 9-2). Indeed these isolates were genotypically identified as P.putida. One strain (P 4-3), identified as, P.alcaligenes based on phenotypic characters, were identified as P.pseudoalcaligenes by 16S rDNA sequencing. These results indicate that the procedures for the identification of Pseudomonas spp. based on phenotypic characteristics should be additionally verified by the molecular methods to obtain results that are meaningful as well as accurate.en_US
dc.language.isoenen
dc.publisherUludağ Üniversitesitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAtıf 4.0 Uluslararasıtr_TR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPseudomonastr_TR
dc.subject16S rDNAtr_TR
dc.subjectCharacterizationen_US
dc.subjectMarineen_US
dc.subjectSDS-PAGEen_US
dc.titleCharacterization of Pseudomonas spp. from seawater of the southwest coast of Turkeyen_US
dc.typeArticleen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.identifier.startpage15tr_TR
dc.identifier.endpage23tr_TR
dc.identifier.volume6tr_TR
dc.identifier.issue16tr_TR
dc.relation.journalJournal of Biological and Environmental Sciencesen_US
Appears in Collections:2012 Cilt 6 Sayı 16

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