Effects of disinfection conditions and culture media on in vitro germination of sea daffodil (Pancratium maritimum)

Abstract

In this study, we investigated the disinfection methods and germination conditions for seeds of sea daffodil (Pancratium maritimum). Seeds were exposed to 20% and 40% NaOCl (Sodium hypochlorite) solutions for 15, 20, and 25 minutes, as disinfection treatments. The rate of 20% NaOCl was the most appropriate concentration for percentage of seed germination (58%) and plant formation (34%). Effects of three culture media on seed germination, plant formation, and growth were investigated: A. Agar (4 gl-1 ) with sucrose (30 gl-1 ); B. MS (Murashige - Skoog) with agar (4 gl-1 ) and sucrose (30 gl-1 ); C. MS with agar (4 gl-1 ), sucrose (30 gl-1 ), and plant growth regulators (1 mgl-1 BA, 0.1 mgl-1 2,4-D). Evaluated plant growth parameters were total plant weight; number, width, and length of leaf; thickness and length of root; and diameter, length, and weight of bulb. All culture media were found to be successful in terms of in vitro germination of the seeds. The most suitable medium for plant formation and all the plant growth parameters were found to be Medium B. This is an innovative report in that it has been thoroughly investigated in terms of disinfection practices and seed germination media with different evaluation parameters.