Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/20932
Title: Immobilization of alpha-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption
Authors: Yavuz, Handan
Denizli, Adil
Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Kimya Bölümü.
Kara, Ali
Osman, Bilgen
Beşirli, Necati
Keywords: Enzyme immobilization
Beads
Alpha-amylase
Chelating beads
N-vinyl imidazole
Methacrylate) membranes
Purification
Affinity adsorbent
Metal
Glucoamylase
Invertase
Chemistry
Engineering
Polymer science
Issue Date: 2005
Publisher: Elsevier
Citation: Kara, A. vd. (2005). "Immobilization of alpha-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption". Reactive & Functional Polymers, 62(1), 61-68.
Abstract: Poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] hydrogel (average diameter 150-200 pm) was prepared by copolymerizing ethylene glycol dimethacrylate (EGDMA) with n-vinyl imidazole (VIM). The copolymer hydrogel bead composition was characterized by elemental analysis and found to contain 5 EGDMA monomer units each VIM monomer unit. Poly(EGDMA-VIM) beads had a specific surface area of 59.8 m(2)/g. Poly- (EGDMA-VIM) beads were characterized by swelling studies and SEM. Cu2+ ions were chelated on the poly(EGDMA-VIM) beads, then these beads were used in the adsorption of alpha-amylase from Aspergillus Oryzae in batch system. The maximum alpha-amylase adsorption capacity of the poly(EGDMA-VIM)-Cu2+ beads was observed as 38.9 mg/g at pH 4.0. The K-m values for immobilized alpha-amylase (poly(EGDMA-VIM)-Cu2+) (22.5 mM) was higher than that of free enzyme (15.8 mM). Storage stability was found to increase with immobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
URI: https://doi.org/10.1016/j.reactfunctpolym.2004.08.008
https://www.sciencedirect.com/science/article/pii/S1381514804001725
http://hdl.handle.net/11452/20932
ISSN: 1381-5148
Appears in Collections:Web of Science

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