Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/21072
Title: Safranin O staining using a microwave oven
Authors: Uludağ Üniversitesi/Tıp Fakültesi/Histoloji-Embriyoloji Anabilim Dalı.
0000-0001-5757-8450
Kahveci, Zeynep
Minbay, Zehra
Çavuşoğlu, İlkin
ABC-1475-2020
Keywords: Biotechnology & applied microbiology
Cell biology
Safranin O
Microwave
Staining method
Light microscopy
Cartilage
Accelerated procedures
Fixation
Irradiation
Microscopy
Artifacts
Sections
Issue Date: 2000
Publisher: Lippincott Williams & Wilkins
Citation: Kahveci, Z. vd. (2000). "Safranin O staining using a microwave oven". Biotechnic & Histochemistry, 75(6), 264-268.
Abstract: We investigated the effects of microwave irradiation on a safranin O staining method for paraffin sections of formalin fixed rabbit larynx. The control sections were stained according to the conventional method, and the experimental sections were stained in microwave oven for 10 sec at 360 W in Weigert's iron hematoxylin, and for 30 sec at 360 W in fast green and 0.1% safranin O staining solutions. Light microscopic examination of the sections revealed that the microwave heating did not adversely affect the staining properties of cartilage tissue compared to the conventional staining method. Small differences such as darker staining of the matrix and shrinkage of the cytoplasm was observed in some microwave treated sections. The present study revealed that microwave application can be used safely for the safranin O method with the advantage of reduced staining time.
URI: https://doi.org/10.3109/10520290009085130
https://www.tandfonline.com/doi/abs/10.3109/10520290009085130
http://hdl.handle.net/11452/21072
ISSN: 1052-0295
Appears in Collections:Web of Science

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