Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/22064
Title: Salmonella profile in chickens determined by real-time polymerase chain reaction and bacteriology from years 2000 to 2003 in Turkey
Authors: Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.
Uludağ Üniversitesi/Yenişehir İbrahim Orhan Meslek Yüksek Okulu.
Eyigör, Ayşegül
Goncagül, Gülşen
Günaydın, Elçin
Carlı, K. Tayfun
E-3867-2010
AAI-1101-2021
AAK-6555-2021
6602558950
8350555500
8350555600
6601971539
Keywords: Tetrathionate broth enrichment
Enterica serotype enteritidis
Environmental-samples
Serogrup-D
PCR
Poultry
Typhimurium
Feces
Identification
Serovars
Veterinary sciences
Aves
Enteritidis
Gallus gallus
Marmara
Meleagris gallopavo
Salmonella
Salmonella enterica
Salmonella enterica subsp. enterica serovar enteritidis
Salmonella enteritidis
Issue Date: Apr-2005
Publisher: Taylor & Francis
Citation: Eyigör, O. vd. (2005). "Salmonella profile in chickens determined by real-time polymerase chain reaction and bacteriology from years 2000 to 2003 in Turkey". Avian Pathology, 34(2), 101-105.
Abstract: From years 2000 to 2003, Salmonella was investigated from a total of 1785 samples comprised of chicken intestinal samples, cloacal swabs, drag swabs, litter samples and chick dust samples collected from 191 poultry breeding flocks belonging to 15 different chicken breeding stock companies in the Marmara region, Turkey by a SYBR green-based real-time polymerase chain reaction (SGBRT-PCR), by a probe-specific real-time polymerase chain reaction (PSRT-PCR) and by standardized bacteriology as described in the manual of National Poultry Improvement Plan and Auxillary Provisions, United States Department of Agriculture. Between January 2000 and July 2001, Salmonella was detected at the rates of 5.87% and 4.10% out of a total of 1242 samples by SGBRT-PCR and bacteriology, respectively. From July 2001 until December 2003, Salmonella was found at rates of 11.42% and 5.52% from a total of 543 samples by PSRT-PCR and bacteriology, respectively. The dominant Salmonella serovar was determined as Salmonella enterica subsp. enterica Serovar Enteritidis ( S. Enteritidis), while serogroup C1 and C2 in 2001 and serogroup E1 in 2002 were isolated as additional serovars. As a conclusion, S. Enteritidis seems to be the major problem in poultry breeding flocks in Turkey, and both of the real-time polymerase chain reaction methods were found more sensitive than standard bacteriology for the detection of Salmonella from poultry samples.
URI: https://doi.org/10.1080/03079450500059461
https://www.tandfonline.com/doi/full/10.1080/03079450500059461
http://hdl.handle.net/11452/22064
ISSN: 0307-9457
1465-3338
Appears in Collections:Scopus
Web of Science



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