Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/22434
Title: The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested
Authors: Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya ve Klinik Kimya Anabilim Dalı.
0000-0002-6729-7908
0000-0003-0463-6818
Ulukaya, Engin
Özdikiçioglu, Ferda
Yılmaztepe, Arzu
Demirci, Meral
AAG-7012-2021
A-5841-2017
K-5792-2018
6602927353
6504081459
23091316500
18534643800
Keywords: MTT assay
ATP assay
Chemotherapeutics
Cyrotoxicity
In vitro
A549
Luminescence assay
Ovarian-cancer
Chemosensitivity assay
Toxiciy assays
Cell-lines
In-vitro
Reduction
Apoptosis
Survival
Cytotoxicity
Issue Date: Feb-2008
Publisher: Pergamon-Elsevier Science
Citation: Ulukaya, E. vd. (2008). ''The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested''. Toxicology in Vitro, 22(1), 232-239.
Abstract: Accurate assessment of the anti-growth effects of chemotherapeutics is immensely importance in cancer research with regard to drug discovery and toxicological safety. A number of in vitro cytotoxicity assays are used for these purposes. However, there is the possibility for different results in the assessments because the way they measure the viability of cancer cells is specific to each assay. In the present study, the performance of two common assays (MTT and ATP) in the assessment of anti-growth effects of chemotherapeutics on a lung cancer cell line (A549) was compared. The cells were treated with paclitaxel, docetaxel, gemcitabine, 5-fluorouracil (5-FU), etoposide, doxorubicin, epirubicin, cisplatin, 4-hydroperoxycyclophosphamide (4-HC) and carboplatin in six different concentrations. When taking all the drugs and inhibitions into account, a moderate correlation (r = 0.671); p = 0.01) between the assays was found. However, IC 50 values by the MTT assay were higher in 90% of the drugs than those found by the ATP assay. In addition to this, there was a statistically significant difference between the dose response curves of the assays, which was dependent on the drugs of choice. We recommend caution in comparing these assays to evaluate the anti-growth effects of chemotherapeutics because the MTT assay seem to give rise to relatively lower inhibition (higher viability) levels than the ATP assay, depending on the drugs of choice.
URI: https://doi.org/10.1016/j.tiv.2007.08.006
https://www.sciencedirect.com/science/article/pii/S0887233307002287
http://hdl.handle.net/11452/22434
ISSN: 08872333
Appears in Collections:Scopus
Web of Science

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