Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/24340
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dc.contributor.authorSeyrek, Kamil-
dc.contributor.authorKıral, Funda Kargın-
dc.contributor.authorMusal, Bayazıt-
dc.contributor.authorToplu, Nihat-
dc.date.accessioned2022-02-02T13:31:01Z-
dc.date.available2022-02-02T13:31:01Z-
dc.date.issued2005-05-
dc.identifier.citationSeyrek, K. vd. (2005). "Biochemical and histochemical detection of the sialic acids in mammary tumours of bitches". Revue de Medecine Veterinaire, 156(5), 258-263.en_US
dc.identifier.issn0035-1555-
dc.identifier.urihttp://hdl.handle.net/11452/24340-
dc.description.abstractThis study describes the expression and localisation of sialic acid in mammary tumours in bitches. Tissue total sialic acid (TSA) concentrations were detected spectrophotometrically. Localisation of sialic acids in mammary tissue was visualised using the biotin-conjugated Sambucus nigra lectin (SNA), specific for alpha 2,6-linked sialic acid, and Maaickia amurensis lectin (MAL), specific for alpha 2,3-linked sialic acid. The mean value of tissue TSA of malignant tumours (1.46 +/- 0.14 mu mol/g protein) was significantly higher (p < 0,001) than that in healthy tissues (0.26 +/- 0.07 mu mol/g protein) as well as in tissues with benign types of tumour (0.50 +/- 0.06 mu mol/g protein). We could not find a significant difference in sialic acid concentrations between adenomas and normal tissue (p > 0.05). Tissue TSA content in the same type of tumours was not always equal. In histochemical analyses both the staining intensity and localisation patterns of SNA and MAL showed marked differences. The staining for SNA and MAL was only low or not present in normal mammary tissues but high in carcinomas. In malign tumours binding sites for SNA were predominantly in epithelial cells and in individual cells infiltrating the fibrous tissue. However, the binding sites for MAL were predominantly in fibrous tissue, capillary and lymphatic endothelia. Contrary to malignant tumours, a moderate staining was observed in benign tumours. The staining was mainly in epithelial cells, in fibrocytes and in myoepithelial cells. It was not always the case that the same type of tumours revealed the same staining intensity localisation for the lectins. The results obtained from the present study suggest that elevated levels of sialic acid in malignant mammary tumours may play an important role in the detachment of carcinoma cells from the primary tumour and would be of critical importance for the metastasis of turnout cells.en_US
dc.language.isoenen_US
dc.publisherEcole Nationale Veterinaire Toulouseen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVeterinary sciencesen_US
dc.subjectSialic aciden_US
dc.subjectMammary tumouren_US
dc.subjectDogen_US
dc.subjectBreast-canceren_US
dc.subjectSerumen_US
dc.subjectExpressionen_US
dc.subjectMarkeren_US
dc.subjectIdentificationen_US
dc.subjectResiduesen_US
dc.subjectLectinen_US
dc.subjectTissueen_US
dc.subjectCanidaeen_US
dc.subjectCanis familiarisen_US
dc.subjectMaackia amurensisen_US
dc.subjectSambucus nigraen_US
dc.titleBiochemical and histochemical detection of the sialic acids in mammary tumours of bitchesen_US
dc.typeArticleen_US
dc.identifier.wos000229924800004tr_TR
dc.identifier.scopus2-s2.0-19944420866tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergien_US
dc.contributor.departmentUludaǧ Üniversitesi/Veterinerlik Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.tr_TR
dc.identifier.startpage258tr_TR
dc.identifier.endpage263tr_TR
dc.identifier.volume156tr_TR
dc.identifier.issue5tr_TR
dc.relation.journalRevue de Medecine Veterinaireen_US
dc.contributor.buuauthorSeyrek, Kamil İntaş-
dc.contributor.buuauthorKeskin, Abdulkadir-
dc.contributor.researcheridAAH-7292-2019tr_TR
dc.relation.collaborationYurt içien_US
dc.subject.wosVeterinary sciencesen_US
dc.indexed.wosSCIEen_US
dc.indexed.scopusScopusen_US
dc.wos.quartileQ4en_US
dc.contributor.scopusid6603409870tr_TR
dc.contributor.scopusid24473229800tr_TR
dc.subject.scopusSialic Acids; Glycoconjugates; Fucoseen_US
dc.subject.emtreeSialic acid derivativeen_US
dc.subject.emtreeAdenomaen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBinding siteen_US
dc.subject.emtreeBreast tumoren_US
dc.subject.emtreeCapillary endotheliumen_US
dc.subject.emtreeCarcinoma cellen_US
dc.subject.emtreeConcentration responseen_US
dc.subject.emtreeConjugationen_US
dc.subject.emtreeEpithelium cellen_US
dc.subject.emtreeGene expressionen_US
dc.subject.emtreeHistochemistryen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeLymphoid tissueen_US
dc.subject.emtreeMetastasisen_US
dc.subject.emtreeMyoepithelium cellen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeSpectrophotometryen_US
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