Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/25030
Title: A comparison of microwave heating and proteolytic pretreatment antigen retrieval techniques in formation fixed, paraffin embedded tissues
Authors: Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
0000-0001-5757-8450
Kahveci, Zeynep
Minbay, Zehra
Noyan, Semiha
Çavuşoǧlu, İlkin
ABC-1475-2020
6603395784
8220935200
56636269700
56009155800
Keywords: Biotechnology and applied microbiology
Cell biology
Animalia
Antigen retrieval
Formalin fixation
Microwave heating
Paraffin sections
Formaldehyde fixation
Formalin
Sections
Oven
Immunohistochemistry
PH
Impregnation
Antibodies
Keratins
Issue Date: Apr-2003
Publisher: Taylor and Francis
Citation: Kahveci, Z. vd. (2003). “A comparison of microwave heating and proteolytic pretreatment antigen retrieval techniques in formation fixed, paraffin embedded tissues”. Biotechnic and Histochemistry, 78(2), 119-128.
Abstract: Antigen retrieval (AR) is a technique that re-exposes epitopes in formalin fixed, paraffin embedded sections and makes them detectable by immunohistochemistry. We compared the effects of two AR procedures, enzyme digestion and microwave heating, on immunostaining of vimentin and desmin in formalin fixed, paraffin embedded tissues. Our results showed that AR is necessary for vimentin and desmin immunostaining in tissues fixed in formalin for more than 48 h. With prolonged fixation times, microwave heating showed better results than enzyme digestion for AR. The same results were obtained using 1% zinc sulfate or Citra Plus solution as retrieval solutions for microwave heating. We recommend microwave heating for AR, because it is easier to use and produces better results compared to enzyme treatment.
URI: https://doi.org/10.1080/10520290310001593775
http://hdl.handle.net/11452/25030
ISSN: 1052-0295
Appears in Collections:Scopus
Web of Science

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