The comparison of the negative effect of autoclaving and pasteurization on bone healing

Abstract

Objectives: The aim of this study was to compare the effects of autoclaving and pasteurization on bone healing.

Methods: Twenty-five full-grown male rabbits were included in the study; all 25 had bone blocks resected and reimplanted. In group 1, bone blocks were autoclaved; in group 2, bone blocks were pasteurized; and in group 3 (controls), resected bone blocks were reimplanted without sterilization.

Results: Heiple scores of the proximal parts of the fusion surfaces in group 1, group 2, and group 3 were 12.8 +/- 0.4, 6.8 +/- 1.2, and 10.2 +/- 1.9, respectively. Heiple scores of the distal parts of the fusion surfaces in group I, group 2, and group 3 were 10.8 +/- 0.8, 6.0 +/- 1.1, and 9.8 +/- 1.5, respectively. Differences in radiologic scores were not statistically significant between the groups for proximal or distal fusion surfaces at 3 and 6 weeks.

Conclusion: In conclusion, pasteurization has a less negative effect on bone healing than autoclaving, and can be considered for bone sterilization in certain circumstances.