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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Adıgüzel, Zelal | - |
dc.contributor.author | Tarık, Ahmet Baykal | - |
dc.contributor.author | Kaçar, Ömer | - |
dc.contributor.author | Açılan, Ceyda | - |
dc.date.accessioned | 2022-09-06T12:31:19Z | - |
dc.date.available | 2022-09-06T12:31:19Z | - |
dc.date.issued | 2014-11 | - |
dc.identifier.citation | Adıgüzel, Z. vd. (2014). "Biochemical and proteomic analysis of a potential anticancer agent: Palladium(II) saccharinate complex of terpyridine acting through double strand break formation". Journal of Proteome Research, 13(11), 5240-5249. | en_US |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.issn | 1535-3907 | - |
dc.identifier.uri | https://doi.org/10.1021/pr5006718 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/pr5006718 | - |
dc.identifier.uri | http://hdl.handle.net/11452/28501 | - |
dc.description.abstract | Metal based chemotherapeutic drugs are widely used as an effective method to defeat various cancers. In this study, the mechanism of action of a novel therapeutic agent, [Pd(sac)(terpy)](sac)center dot 4H(2)O (sac = saccharinate, and terpy = 2,2':6',2 ''-terpyridine) was studied. To better understand the proteomic changes in response to this agent, we performed nano LC-MS/MS analyses in human breast cancer cells (MDA-MB-231). Thirty proteins were identified to be differentially expressed more than 40% after drug treatment. Many cellular pathways were affected, including proteins involved in DNA repair, apoptosis, energy metabolism, protein folding, cytoskeleton, pre-mRNA maturation, or protein translation. The changes in protein expression were further verified for XRCC5, which plays a role in double strand break (DSB) repair, and ubiquitin, which is involved in protein degradation and apoptosis. The elevated XRCC5 levels were suggestive of increased DSBs. The presence of DSBs was confirmed by smearing of plasmid DNA in vitro and induction of gamma H2AX foci in vivo. There was also increased intracellular reactive oxygen species (ROS) formation, as detected by 2',7'-dichlorofluorescein diacetate (DCFDA) staining. Scavenging ROS by N-acetylcysteine rescued cell death in response to Pd(II) treatment, potentially explaining how the Pd(II) complex damaged the DNA. The details of this analysis and the significance will be discussed during the scope of this work. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Amer Chemical | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Palladium(II) saccharinate complex with terpyridine | en_US |
dc.subject | Anticancer drugs | en_US |
dc.subject | DNA double strand breaks | en_US |
dc.subject | Reactive oxygen species | en_US |
dc.subject | Nonhomologous end joining | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Proteomics | en_US |
dc.subject | Cancer-cells | en_US |
dc.subject | Statistical-model | en_US |
dc.subject | Platinum(II) | en_US |
dc.subject | Proteins | en_US |
dc.subject | Biochemistry & molecular biology | en_US |
dc.subject.mesh | Antineoplastic agents | en_US |
dc.subject.mesh | Breast neoplasms | en_US |
dc.subject.mesh | Cell line, tumor | en_US |
dc.subject.mesh | Coordination complexes | en_US |
dc.subject.mesh | DNA breaks, double-stranded | en_US |
dc.subject.mesh | DNA helicases | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | HeLa cells | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Palladium | en_US |
dc.subject.mesh | Proteomics | en_US |
dc.subject.mesh | Reactive oxygen species | en_US |
dc.subject.mesh | Tandem mass spectrometry | en_US |
dc.title | Biochemical and proteomic analysis of a potential anticancer agent: Palladium(II) saccharinate complex of terpyridine acting through double strand break formation | en_US |
dc.type | Article | en_US |
dc.identifier.wos | 000344636500065 | tr_TR |
dc.identifier.scopus | 2-s2.0-84908868909 | tr_TR |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı. | tr_TR |
dc.contributor.orcid | 0000-0002-2849-3332 | tr_TR |
dc.identifier.startpage | 5240 | tr_TR |
dc.identifier.endpage | 5249 | tr_TR |
dc.identifier.volume | 13 | tr_TR |
dc.identifier.issue | 11 | tr_TR |
dc.relation.journal | Journal of Proteome Research | en_US |
dc.contributor.buuauthor | Yılmaz, Veysel Turan | - |
dc.contributor.buuauthor | Ulukaya, Engin | - |
dc.contributor.researcherid | K-5792-2018 | tr_TR |
dc.contributor.researcherid | L-7238-2018 | tr_TR |
dc.relation.collaboration | Yurt içi | tr_TR |
dc.relation.collaboration | Sanayi | tr_TR |
dc.identifier.pubmed | 25210790 | tr_TR |
dc.subject.wos | Biochemical research methods | en_US |
dc.indexed.wos | SCIE | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.pubmed | PubMed | en_US |
dc.wos.quartile | Q1 | en_US |
dc.contributor.scopusid | 7006269202 | tr_TR |
dc.contributor.scopusid | 6602927353 | tr_TR |
dc.subject.scopus | Complex; Palladium; 2-Phenylpyridine | en_US |
dc.subject.emtree | Acetylcysteine | en_US |
dc.subject.emtree | Antineoplastic agent | en_US |
dc.subject.emtree | Cisplatin | en_US |
dc.subject.emtree | Histone H2AX | en_US |
dc.subject.emtree | Messenger RNA precursor | en_US |
dc.subject.emtree | Palladium complex | en_US |
dc.subject.emtree | Palladium saccharinate | en_US |
dc.subject.emtree | Plasmid DNA | en_US |
dc.subject.emtree | Reactive oxygen metabolite | en_US |
dc.subject.emtree | Saccharin sodium | en_US |
dc.subject.emtree | Ubiquitin C | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | XRCC5 protein | en_US |
dc.subject.emtree | Antineoplastic agent | en_US |
dc.subject.emtree | Coordination compound | en_US |
dc.subject.emtree | DNA helicase | en_US |
dc.subject.emtree | Palladium | en_US |
dc.subject.emtree | Reactive oxygen metabolite | en_US |
dc.subject.emtree | Saccharinate(2,2'-6',2'-terpyridine)palladium(II) | en_US |
dc.subject.emtree | XRCC5 protein | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Apoptosis | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Biochemistry | en_US |
dc.subject.emtree | Breast cancer cell line | en_US |
dc.subject.emtree | Cell death | en_US |
dc.subject.emtree | Cell viability | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Cytoskeleton | en_US |
dc.subject.emtree | DNA repair | en_US |
dc.subject.emtree | Double stranded DNA break | en_US |
dc.subject.emtree | Drug mechanism | en_US |
dc.subject.emtree | Energy metabolism | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | In vitro study | en_US |
dc.subject.emtree | In vivo study | en_US |
dc.subject.emtree | Liquid chromatography | en_US |
dc.subject.emtree | Nucleotide sequence | en_US |
dc.subject.emtree | Oxidative stress | en_US |
dc.subject.emtree | Protein analysis | en_US |
dc.subject.emtree | Protein degradation | en_US |
dc.subject.emtree | Protein expression | en_US |
dc.subject.emtree | Protein folding | en_US |
dc.subject.emtree | Proteomics | en_US |
dc.subject.emtree | Tandem mass spectrometry | en_US |
dc.subject.emtree | Breast neoplasms | en_US |
dc.subject.emtree | Chemistry | en_US |
dc.subject.emtree | Double stranded DNA break | en_US |
dc.subject.emtree | Drug effects | en_US |
dc.subject.emtree | Female | en_US |
dc.subject.emtree | HeLa cell line | en_US |
dc.subject.emtree | Metabolism | en_US |
dc.subject.emtree | Pathology | en_US |
dc.subject.emtree | Procedures | en_US |
dc.subject.emtree | Proteomics | en_US |
dc.subject.emtree | Tumor cell line | en_US |
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