Please use this identifier to cite or link to this item:
http://hdl.handle.net/11452/28721
Title: | RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North America |
Authors: | Maan, Narender Singh Maan, Sushila Nomikou, Kyriaki Johnson, Donna J. El-Harrak, Mehdi Madani, Hafsa Yadin, Hagai İnçoğlu, Şerife Allison, Andrew B. Stallknecht, David E. Batten, Carrie Anne Anthony, Simon John Mertens, Peter Paul Clement Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Öncesi Bilimler Bölümü. Yeşilbağ, Kadir ABE-7662-2020 6602912127 |
Keywords: | White-tailed deer Polymerase chain-reaction Bluetongue-virus Competitive elisa Ibaraki-virus Sequence-analysis Genome Differentiation Antibodies Proteins Science & technology - other topics Bos Epizootic hemorrhagic disease virus Mammalia Miridae |
Issue Date: | 17-Sep-2010 |
Publisher: | Public Library Science |
Citation: | Maan, N. S. vd. (2010). "RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the mediterranean region and North America". Plos One, 5(9), 1-13. |
Abstract: | Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genomesegment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America. |
URI: | https://doi.org/10.1371/journal.pone.0012782 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0012782 http://hdl.handle.net/11452/28721 |
ISSN: | 1932-6203 |
Appears in Collections: | Scopus Web of Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Yeşilbağ_vd_2010.pdf | 2.93 MB | Adobe PDF | ![]() View/Open |
This item is licensed under a Creative Commons License