Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/29527
Title: Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
Authors: Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.
Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.
0000-0001-5141-0008
0000-0002-7678-3289
0000-0002-9634-0055
0000-0002-8872-0074
Alçay, Selim
Gökçe, Elif
Toker, M. Berk
Önder, N. Tekin
Üstüner, Burcu
Uzabacı, Ender
Gül, Zülfiye
Çavuş, Seda
AAF-9939-2020
AAG-7238-2021
56099810300
56779799700
56480349200
55670728900
18937724600
55347697800
56086542900
57188685710
Keywords: Life sciences & biomedicine - other topics
Physiology
Freeze-drying
Goat semen
Cryopreservation
Antioxidants
Incubation resilience
Ram semen frozen
Soybean lecithin
Sperm parameters
Cryopreservation
Fertility
Integrity
Acrosome
Agents
Issue Date: 21-Mar-2016
Publisher: Elsevier
Citation: Alçay, S. vd. (2016). "Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa". Cryobiology, 72(3), 269-273.
Abstract: The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.
URI: https://doi.org/10.1016/j.cryobiol.2016.03.007
https://www.sciencedirect.com/science/article/pii/S0011224016300189
http://hdl.handle.net/11452/29527
ISSN: 0011-2240
1090-2392
Appears in Collections:Scopus
Web of Science

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