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Title: | Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of Lactococcus garvieae Strains |
Authors: | Onuk, Ertan Emek Çiftçi, Alper Uludağ Üniversitesi/Veterinerlik Fakültesi/Su Hayvanları Hastalıkları Bölümü. 0000-0001-9096-875X 0000-0001-7707-2705 Altun, Soner Büyükekiz, Ayşe Gül Duman, Muhammed AAG-8518-2021 T-1697-2019 56269221600 55567777200 55568071100 |
Keywords: | Veterinary sciences Oncorhynchus mykiss Lactococcus garvieae Rapid32 STREP RAPD PCR Antimicrobial sensitivity Trout oncorhynchus-mykiss Polymorphic dna analysis Cultured marine fish Genetic-characterization Enterococcus-seriolicida Countries Synonym Genomes Europe Turkey |
Issue Date: | 2013 |
Publisher: | Kafkas Üniversitesi |
Citation: | Altun, S. vd. (2013). "Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of Lactococcus garvieae Strains". Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 375-381. |
Abstract: | In this study, phenotypic and genotypic features of 10 L. garvieae strains isolated from rainbow trout farms were examined with 3 reference strains (Spain, England and ATCC 43921) comperativly. Rapid 32 STREP and conventional microbiologic tests were used for determining phenotipic features of L. garvieae strains. Altough there are differences, in Rapid 32 STREP system, between strains in terms of beta-Glucuronidase, D-ribose, sorbitol, lactose, raffinose, alanyl-phenylalanyl-proline arylamidase, pyrrolidonyl arylamidase, hippurate hydrolysis, urease tests, all strains have been confirmed as L. garvieae by API web. In RAPD PCR analysis, in which ERIC 2 primer is used, L. garvieae isolates were genotyped within 3 separate clusters according to similarity coefficient index of 70%, and it was detected that a vast majority of isolates with Turkey-origin (8 isolates) belongs to predominant type LG1 genotype. In addition to this, antimicrobial tests of L. garvieae strains shows that there are resistance against gentamycin, neomycin, lincomycin, sulfamethoxazole-trimethoprim, oxytetracycline, erythromycin, amoxicillin, florfenicol and doxycycline, which are frequently used on fish in our country. |
URI: | https://doi.org/10.9775/kvfd.2012.7754 https://vetdergikafkas.org/abstract.php?id=1334 https://vetdergikafkas.org/uploads/pdf/pdf_KVFD_1334.pdf http://hdl.handle.net/11452/29683 |
ISSN: | 1300-6045 |
Appears in Collections: | Scopus TrDizin Web of Science |
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