Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/30041
Title: Immunohistochemical evidence for glutamatergic regulation of nesfatin-1 neurons in the rat hypothalamus
Authors: Serter Kocaoğlu, Sema
Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
0000-0001-5757-8450
0000-0003-3463-7483
Gök, Duygu Yurtseven
Minbay, Zehra
Ezigör, Özhan
ABE-5128-2020
ABC-1475-2020
AAW-4867-2021
57193760779
8220935200
6603109907
Keywords: Glutamate
Nesfatin-1
C-Fos
Hypothalamus
Rat
Blood-brain-barrier
Subunit messenger-RNAs
C-fos
Receptor subunits
Ultrastructural-localization
Satiety molecule
Kainic acid
Expression
Kainate
Activation
Neurosciences & neurology
Issue Date: 7-Sep-2020
Publisher: MDPI
Citation: Yurtseven, D. G. vd. (2020). "Immunohistochemical evidence for glutamatergic regulation of nesfatin-1 neurons in the rat hypothalamus". Brain Sciences, 10(9).
Abstract: Nesfatin-1, identified as an anorexigenic peptide, regulates the energy metabolism by suppressing food intake. The majority of nesfatin-1-synthesizing neurons are concentrated in various hypothalamic nuclei, especially in the supraoptic (SON), arcuate (ARC) and paraventricular nuclei (PVN). We tested the hypothesis that the glutamatergic system regulates nesfatin-1 neurons through glutamate receptors. Therefore, the first aim of the proposed studies was to examine effects of different glutamate agonists in the activation of nesfatin-1 neurons using c-Fos double immunohistochemical labeling. Experimental groups were formed containing male and female rats which received intraperitoneal injections of glutamate agonists kainic acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) while the control rats received vehicle. The significant increase in the number of c-Fos-expressing nesfatin-1 neurons after agonist injections were observed both in female and male subjects and some of these effects were found to be sexually dimorphic. In addition, treatment with specific glutamate antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) or dizocilpine (MK-801) before each of the three agonist injections caused a statistically significant reduction in the number of activated nesfatin-1 neurons in the hypothalamic nuclei including supraoptic, paraventricular and arcuate nuclei. The second aim of the study was to determine the expression of glutamate receptor subunit proteins in the nesfatin-1 neurons by using a double immunofluorescence technique. The results showed that the glutamate receptor subunits, which may form homomeric or heteromeric functional receptor channels, were expressed in the nesfatin-1 neurons. In conclusion, the results of this study suggest that nesfatin-1 neurons respond to glutamatergic signals in the form of neuronal activation and that the glutamate receptors that are synthesized by nesfatin-1 neurons may participate in the glutamatergic regulation of these neurons.
URI: https://doi.org/10.3390/brainsci10090630
https://www.mdpi.com/2076-3425/10/9/630
http://hdl.handle.net/11452/30041
ISSN: 2076-3425
Appears in Collections:PubMed
Scopus
Web of Science

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