Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/30275
Title: Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors
Authors: Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
Uludağ Üniversitesi/Veteriner Fakültesi.
0000-0002-5090-7917
Akkoç, Ahmet
Nak, Deniz
Demirer, Aylin
Şimşek, Gözde
55584229300
9280090000
54402527300
26435497200
Keywords: Biotechnology & applied microbiology
Cell biology
Canine
Dog
Immunohistochemistry
Matrix metalloproteinase
Venereal tumor
Expression
Dogs
Metastasis
Sarcoma
Cancer
Brain
Mmp-2
Issue Date: 7-Nov-2016
Publisher: Taylor & Francis
Citation: Akkoç, A. vd. (2017). ''Immunocharacterization of matrix metalloproteinase-2 and matrix metalloproteinase-9 in canine transmissible venereal tumors''. Biotechnic and Histochemistry, 92(2), 100-106.
Abstract: Matrix metalloproteases (MMPs) are endogenous proteases that are responsible for degradation of extracellular matrix (ECM) proteins and cell surface antigens. The breakdown of ECM participates in the local invasion and distant metastases of malignant tumors. Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious round cell neoplasm of dogs that affects mainly the external genitalia of both sexes. CTVT generally is a locally invasive tumor, but distant metastases also are common in puppies and immunocompromised dogs. We investigated the immune expressions and activities of MMP-2 and MMP-9 in CTVT. The presence of these enzymes in tumor cells and tissue homogenates was demonstrated by immunohistochemistry and western blotting. We used gelatin substrate zymography to evaluate the activities of MMP-2 and MMP-9 enzymes in tumor homogenates. We found that tumor cells expressed both MMP-2 and MMP-9. Electrophoretic bands corresponding to MMP-9 and MMP-2 were identified in immunoblots and clear bands that corresponded to the active forms of MMP-2 and MMP-9 also were detected in gelatin zymograms. Our study is the first detailed documentation of MMPs in CTVT.
URI: https://doi.org/10.1080/10520295.2016.1259500
https://www.tandfonline.com/doi/full/10.1080/10520295.2016.1259500
1473-7760
http://hdl.handle.net/11452/30275
ISSN: 1052-0295
Appears in Collections:Scopus
Web of Science

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