Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/30375
Title: The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts
Authors: Karakaş, Didem
Ulukaya, Engin
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
0000-0002-6729-7908
Arı, Ferda
AAG-7012-2021
24376085300
Keywords: Life sciences & biomedicine - other topics
ATP assay
Breast cancer
Cytotoxicity
Interference
MTT assay
Plant extract
Rapid colorimetric assay
Tetrazolium salt
Cyto-toxicity
In-vitro
Reduction
Growth
Atp
Proliferation
Lines
Survival
Issue Date: 2017
Publisher: TÜBİTAK
Citation: Karakaş, D. vd. (2017). ''The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts''. Turkish Journal of Biology, 41(6), 919-925.
Abstract: The MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plants are used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study, the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants (Hypericum adenotrichum, Salvia kronenburgii, and Pelargonium quercetorum) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231) using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to its interference-free feature. Additionally, decreased cell density was confirmed with phase-contrast microscopy and fluorescence staining (Hoechst 33342 dye). Although both of the viability/cytotoxicity assays are considered as metabolic assays, viabilities (in %) in the MTT assay were found to be strikingly higher when compared to the results with the ATP assay. Even in the case of total death, the MTT assay still produced artificial/false increases in viability. The morphology-based evaluation of viability/cytotoxicity by phase-contrast microscopy and Hoechst 33342 staining were greatly compatible with the ATP assay results. Overestimated (false) viabilities in the MTT assay suggests a serious interference between the MTT assay itself and the extracts used. Some ingredients of plants may have reducing activity (like the dehydrogenase activity of the cells) that converts the MTT compound into the colored formazan that is the principle of the assay. Therefore, the MTT assay may not be a suitable assay for some plant extracts, urging great caution when plants are used
URI: https://doi.org/10.3906/biy-1703-104
https://journals.tubitak.gov.tr/biology/vol41/iss6/7/
1303-6092
http://hdl.handle.net/11452/30375
ISSN: 1300-0152
Appears in Collections:Scopus
TrDizin
Web of Science

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