Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/30652
Title: Histologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosis
Other Titles: Nöroanastomoz sonrası stromal vasküler fraksiyonun nörorejeneratif etkisinin histolojik ve elektromiyografik değerlendirilmesi
Authors: Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.
Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.
0000-0001-5472-9065
0000-0003-0154-9938
0000-0001-9650-0891
0000-0001-5323-6891
000-0003-1547-7293
0000-0001-6548-8754
Acar, Hilal
Çerçi, Ece
Khezri, Marzieh Karimi
Çetin, Melike
Canatan, Uygur
Şen, Mehmet Metin
Aslan, Vildan
Sarıl, Canan Altıncı
Temiz, Elif Mekik
Taşkapılıoğlu, Mevlüt Özgür
Erdost, Hatice
Salcı, Hakan
AAH-9216-2021
AAI-6861-2021
AAW-5254-2020
ABB-8161-2020
57209653860
57212212053
57216947212
56393942900
57189443508
57216950078
57200994296
57216954758
57216945905
25936798300
6505787570
8680329000
Keywords: Electromyography
Histology
Neuroanastomosis
Rat
Stromal vascular fraction
Peripheral-nerve regeneration
Fibrin glue
Hyaluronic-acid
Stem-cells
Repair
Rat
Tissue
Suture
Defect
Veterinary sciences
Issue Date: 23-Mar-2020
Publisher: Kafkas Üniversitesi
Citation: Acar, H. vd. (2020). "Histologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosis". Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(4), 483-490.
Abstract: In this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed. Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7). Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method. The left sciatic nerve of all rats was evaluated for EMG as control. Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture. EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8th weeks. EMG results between and within the groups were statistically evaluated by one-way analysis of variance. Rats in all groups were sacrificed by decapitation at PO 8th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues. EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8th week. Amplitude and conduction velocity increased gradually in all groups. In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8th week. Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8th week in all groups (P>0.05). In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively. In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV. As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.
URI: https://doi.org/10.9775/kvfd.2019.23576
https://vetdergikafkas.org/uploads/pdf/pdf_KVFD_2697.pdf
http://hdl.handle.net/11452/30652
ISSN: 1300-6045
Appears in Collections:PubMed
Scopus
TrDizin
Web of Science

Files in This Item:
File Description SizeFormat 
Acar_vd_2020.pdf2.92 MBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.