Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/30918
Title: Effects of adrenomedullin and glucagon-like peptide on distal flap necrosis and vascularity: The role of receptor systems and nitric oxide
Authors: Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi.
Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Biyofizik Anabilim Dalı.
Cam, Betül
Bağdaş, Deniz
Özyiğit, Musa Özgür
Sağdilek, Engin
Büyükcoşkun, Naciye İsbil
Özlük, Kasım
AAH-1692-2021
AAH-2873-2021
AAR-6478-2021
AAH-4397-2021
56427863700
15062425700
6507338060
16835756600
6603128152
6602676331
Keywords: Dermatology
Surgery
Flap necrosis
Adrenomedullin
Glucagon-like peptide-1
Angiogenesis
Endothelial growth-factor
A signaling pathway
Cell-proliferation
Protein-kinase
Skin flaps
Beta-cells
Rat model
Angiogenesis
Apoptosis
Survival
Issue Date: Jun-2017
Publisher: H. M. P. Commjnications
Citation: Cam, B. vd. (2017). ''Effects of adrenomedullin and glucagon-like peptide on distal flap necrosis and vascularity: The role of receptor systems and nitric oxide''. Wounds, 29(6), 163-167.
Abstract: Objective. Flap necrosis in the distal area due to the deficiency of blood circulation is a major complication in flap treatment. In many previous studies, some natural substances such as chlorogenic acid, adrenomedullin (ADM), and glucagon-like peptide-1 (GLP-1) have been used to improve flap viability via their vasodilator, angiogenic, and antioxidant effects. The aim of this study is to clarify the mechanism through the use of selective antagonists for calcitonin gene-related peptide (CGRP) receptors and GLP-1,receptors such as CGRP-(8-37), exendin-(9-39), respectively, in the flap healing effects of ADM and GLP-1. The role of nitric oxide (NO) was investigated in the mechanism as well. Materials and Methods. Seventy adult female Wistar rats (200 g-250 g) were used in the study. The cutaneous skin flap (8 cm x 3 cm) on the abdominal wall was raised based on the superficial inferior epigastric artery (SIEA). Single-dose substance injections were administered into the SIEA. Necrosis in the flap area was evaluated on postoperative day 7. The proportion of the necrosis area (necrosis area % = [necrosis area/ flap area] x 100) and vascularity (vascular number/cm(2)) in the distal area were calculated. Results. The administrations of ADM or GLP-1 increased the vascularity and decreased the necrosis area in the distal flap region. The ADM receptor antagonist, CGRP-(8-37), did not prevent the positive effects of ADM on flap healing and vascularity. A GLP-1 receptor antagonist, exendin-(9-39), prevented the effect of GLP-1 on flap healing and vascularity. Nitric oxide mediated the beneficial effects of both peptides on flap healing. Conclusion. The CGRP receptors have no direct role, but NO acts as a mediator in the beneficial effect of ADM on flap healing. The GLP-1 specific receptors and NO act as important interagents for the effects of GLP-1 on flap healing.
URI: 1943-2704
http://hdl.handle.net/11452/30918
ISSN: 1044-7946
Appears in Collections:Scopus
Web of Science

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