Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/31293
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dc.date.accessioned2023-03-02T10:03:04Z-
dc.date.available2023-03-02T10:03:04Z-
dc.date.issued2022-09-12-
dc.identifier.citationGüzel, S. (2022). ''Changes in the gene expression of pyruvate dehydrogenase kinase isoenzymes during early differentiation of mouse embryonic stem cells''. Veteriner Hekimlikte Araştırma Dergisi, 41(2), 92-98.tr_TR
dc.identifier.issn1301-3173-
dc.identifier.urihttps://doi.org/10.30782/jrvm.1131148-
dc.identifier.urihttps://dergipark.org.tr/tr/download/article-file/2488176-
dc.identifier.urihttp://hdl.handle.net/11452/31293-
dc.description.abstractThe embryonic stem cells (ESCs) are pluripotent, self-renewing cells that are able to differentiate into any of the germ layers involved in embryogenesis. However, the molecular mechanisms that control ESC pluripotency and differentiation remain poorly understood. The family of Pyruvate dehydrogenase kinase (PDK1-4), inactivates the mitochondrial pyruvate dehydrogenase complex via phosphorylation, plays a crucial role in the control of glucose homeostasis. In the current study, gene expression levels of PDK isoenzymes were analyzed on undifferentiated mouse embryonic stem cells (mESCs) and compared to mESCs induced to differentiate by removal of leukemia inhibitory factor (LIF) for 1, 3 and 5 days. Besides, gene expression analysis of several genes related to pluripotency and differentiation were performed by real-time quantitative PCR. In addition, glucose uptake rates in early differentiated and undifferentiated mESCs were determined using a colorimetric assay kit. Differently expression level of PDK isoenzymes in pluripotent and LIF-depleted mESCs suggest that they may have roles in differentiation and pluripotency of ESCs. Furthermore, this study lays the foundation for detailed investigation of molecular mechanisms underlying the roles of PDKs in the pluripotency and transition to differentiated state of ESCs.en_US
dc.language.isoenen_US
dc.publisherBursa Uludağ Üniversitesitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAtıf 4.0 Uluslararasıtr_TR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectStem cellen_US
dc.subjectPyruvate dehydrogenase kinaseen_US
dc.subjectDifferentiationen_US
dc.titleChanges in the gene expression of pyruvate dehydrogenase kinase isoenzymes during early differentiation of mouse embryonic stem cellsen_US
dc.typeArticleen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.contributor.orcid0000-0003-0796-5000tr_TR
dc.identifier.startpage92tr_TR
dc.identifier.endpage98tr_TR
dc.identifier.volume41tr_TR
dc.identifier.issue2tr_TR
dc.relation.journalVeteriner Hekimlikte Araştırma Dergisi / Journal of Research in Veterinary Medicinetr_TR
dc.contributor.buuauthorGüzel, Saime-
Appears in Collections:2022 Cilt 41 Sayı 2

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