Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/33495
Title: Differential expression and localization of Nanog, Oct 3/4 and C-kıt in mouse ovarian tissue according to age
Authors: Abban, Mete, Gulcin
Dodurga, Yavuz
Satıroglu, Tufan Naciye Lale
Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
Gök, Duygu Yurtsever
AAW-4867-2021
57193446960
Keywords: General & internal medicine
Immunohistochemistry
Ovary
Stem cells
Germ-cells
Stem-cells
Identification
Pluripotency
Genes
Roles
Line
SOX2
Issue Date: Mar-2017
Publisher: Kuwait Medicana Assoc
Citation: Gök, Y. D. vd. (2017). ''Differential expression and localization of Nanog, Oct 3/4 and C-kıt in mouse ovarian tissue according to age''. Kuwait Medical Journal, 49(1), 29-39.
Abstract: Objective: To investigate the expression of embryonic/pluripotent stem cell markers including Nanog, Octamer Binding Protein (3)/(4) (Oct 3/4) and c-Kit from the newborn to aging period in the ovary tissues of mouse. Design: Experimental study using mouse ovary tissues. The expression and localization of Nanog, Oct 3/4 and c-Kit expression were studied in newborn, pubertal, adult and aging ovary. Setting: Department of Histology and Embryology, Pamukkale University, School of Medicine, Turkey Subjects: Newborn (n = 6), pubertal (38-day-old) (n = 6), adult (12-week-old) (n = 6) and aged (24-week-old) female Balb/c mice were used in this study. Intervention : No intervention Main Outcome Measure: The expression of Nanog, Oct 3/4 and c-kit was evaluated by immunohistochemistry and reverse transcriptase chain reaction (PCR). Results: Nanog, Oct 3/4 and c-Kit expression were positive in oocytes of newborn, pubertal and adult ovary. But they were negative in granulosa cells in newborn groups. The expression of these markers in adult period was increased. In addition, positive reaction for Nanog, Oct 3/4 and c-Kit was observed in granulosa cells in secondary and tertiary follicles in pubertal and adult ovary. Ovarian surface epithelium were positive for all stem cells markers in adult and aged. In addition to that, only c-kit positive expression was found in theca cells. Conclusion: According to our findings, each of the three stem cell markers may play an important role in folliculogenesis and ovarian pathology. However, c-kit may be more effective than others because stromal cells were positive in adult and pubertal ovaries as well as in aged ovary.
URI: http://hdl.handle.net/11452/33495
ISSN: 0023-5776
Appears in Collections:Scopus
Web of Science

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