Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/34101
Title: Application of affinity microspheres for effective SPE cleanup before the determination of sulfamerazine by HPLC
Authors: Kılıç, Gözde
Tüzmen, Nalan
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Fizik Bölümü.
Osman, Bilgen
ABF-4791-2020
15221651200
Keywords: Materials science
Affinity microspheres
Antibiotic
Sulfamerazine
SPE cleanup
HPLC
Solid-phase extraction
Performance liquid-chromatography
Personal care products
Aqueous-solution
Mass-spectrometry
Malachite-green
Estrogenic chemicals
Antibiotic-residues
Confirmatory method
Activated carbon
Amino acids
Antibiotics
Chromatographic analysis
Desorption
Esters
Ethylene
Ethylene glycol
Ionic strength
High performance liquid chromatography
Phase separation
Polyols
Adsorption capacities
Ethylene glycol dimethacrylate
HPLC
Optimized conditions
Solid-phase extraction
SPE clean-up
Sulfamerazine
Suspension polymerization
Microspheres
Issue Date: 19-Apr-2018
Publisher: Elsevier
Citation: Kılıç, G. vd. (2018). ''Application of affinity microspheres for effective SPE cleanup before the determination of sulfamerazine by HPLC''. Materials Science and Engineering C., 91, 55-63.
Abstract: This paper describes the application of poly (ethylene glycol dimethacrylate-N-methacryloyl-L-tryptophane methyl ester) [p(EGDMA-MATrp)] microspheres as an affinity sorbent for the SPE (solid phase extraction) cleanup of sulfamerazine (SMR) from food samples of animal origin before high performance liquid chromatography (HPLC) analysis. The microspheres were prepared by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl-L-tryptophane methyl ester (MATrp) as a crosslinker and a monomer, respectively. Various parameters affecting the SPE cleanup efficiency of the p(EGDMA-MATrp) microspheres (contact time, pH, initial SMR concentration, ionic strength etc.) were optimized. Under the optimized conditions, maximum adsorption capacity was found to be 8.55 +/- 0.44 mg/g sorbent at pH 5.0. 90% of the adsorbed SMR was desorbed by using ACN:MeOH (5:5) mixture as a desorption agent. Applicability of the microspheres for the SPE cleanup of SMR residues in the food samples such as egg and milk with HPLC was also determined. It was demonstrated that the prepared p(EGDMA-MATrp) microspheres could be repeatedly applied for SPE cleanup of sulfamerazine before chromatographic analysis. Also, the recoveries of SMR in milk and egg samples were reasonably satisfactory and in the range of 71 to 90%.
URI: https://doi.org/10.1016/j.msec.2018.04.058
https://www.sciencedirect.com/science/article/pii/S0928493117342960
http://hdl.handle.net/11452/34101
ISSN: 0928-4931
1873-0191
Appears in Collections:Scopus
Web of Science

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