Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/34829
Title: Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo
Authors: Gürel, Ebru Gürevin
Kıyan, Hülya Tuba
Esener, Osman Behzat Burak
Üvez, Ayca
Ulukaya, Engin
Dimas, Konstantinos
Armutak, Elif İlkay
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
0000-0001-5238-2432
Aydınlık, Şeyma
ABI-2909-2020
57190280044
Keywords: Oncology
Doxorubicin
Chloroquine
Angiogenesis
CAM assay
Tube formation
Chorioallantoic membrane
Breast-cancer
Cell-line
Doxorubicin
Therapy
Model
Inhibition
Efficacy
Cardiotoxicity
Nanoparticles
Issue Date: 21-May-2018
Publisher: Int Inst Anticancer Research
Citation: Gürel, G. E. vd. (2018). ''Chloroquine used in combination with chemotherapy synergistically suppresses growth and angiogenesis in vitro and in vivo''. Anticancer Research, 38(7), 4011-4020.
Abstract: Background: The inhibition of autophagy using pharmacological inhibitors such as chloroquine may be an effective strategy to overcome chemotherapy or resistance to anti-angiogenic therapy. Materials and Methods: The cytotoxic effect of doxorubicin (0.1-1 mu M), chloroquine (0.25-32 ,mu M) and their combination were investigated by employing ATP assay in human umbilical vein endothelial cells (HUVECs). The effect of doxorubicin and chloroquine combination was also measured using tube formation assay on Matrigel. The anti-angiogenic activities of doxorubicin (2.5 mu g/pellet) and chloroquine (15 mu g/pellet), their combination, and standards (50 mu g/pellet) were tested in vivo using the chick embryo chorioallantoic membrane (CAM) assay. Results: The combination of doxorubicin and chloroquine significantly had a stronger anti-angiogenic effect than the positive control (+/-)-thalidomide and doxorubicin alone in the CAM assay and in vitro tube-formation assay. Conclusion: Chloroquine enhanced the anti-angiogenic effect of doxorubicin on CAM at the tested concentrations.
URI: https://doi.org/10.21873/anticanres.12689
https://ar.iiarjournals.org/content/38/7/4011
http://hdl.handle.net/11452/34829
ISSN: 0250-7005
1791-7530
Appears in Collections:Scopus
Web of Science

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