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Başlık: Carnation etched ring virus elimination through shoot tip culture
Yazarlar: Ashnayi, Mahnaz
Kharrazi, Mahdiyeh
Sharifi, Ahmad
Mehrvar, Mohsen
Anahtar kelimeler: Carnation etched ring virus
PCR
Meristem culture
DAS-ELISA
Yayın Tarihi: 2012
Yayıncı: Uludağ Üniversitesi
Atıf: Ashnayi, M. vd. (2012). "Carnation etched ring virus elimination through shoot tip culture". Journal of Biological and Environmental Sciences, 6(17), 175-180.
Özet: Carnation etched ring virus (CERV) is the second most destructive virus which infects carnation and the only DNA virus among infecting viruses of carnation. In symptomatic leaves of carnation consist of mottling, necrotic and chlorotic flecks or blotches. Virus was detected by DAS-ELISA and PCR. Treatments consisted of different sizes of meristem and MS medium supplemented with different plant growth regulators (PGRS) (0.5 mg/l benzyl adenine (BA), 0.5 mg/l gibberellic acid (GA3) and medium without PGRS.). The plantlets were analysed by PCR in order to evaluate virus eradication. Results of PCR in vitro culture revealed that explant size and type of PGRS had a significant effect on elimination of CERV and the highest amount of it (100%) was observed on medium containing BA in meristem size of 0.4, 0.7 mm and the lowest amount of it (26%) was occurred on medium supplemented with GA3 in meristem size of 1mm. So far, there is no reporting about influence of PGRS on elimination of viruses.
URI: https://dergipark.org.tr/tr/download/article-file/497519
http://hdl.handle.net/11452/17743
ISSN: 1307-9530
1308-2019
Koleksiyonlarda Görünür:2012 Cilt 6 Sayı 17

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