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http://hdl.handle.net/11452/22629
Başlık: | Rapid polymerisation with microwave irradiation for transmission electron microscopy |
Yazarlar: | Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı. 0000-0001-5757-8450 Çavuşoğlu, İ. Minbay, Zehra Şehime, Temel ABC-1475-2020 AAG-8385-2021 56009155800 8220935200 6507885442 56636269700 |
Anahtar kelimeler: | Electron microscopy Epon Microwave irradiation Polymerisation Ultrathin Sections Preservation Embedment Fixatıon Light Animalia Anatomy & morphology |
Yayın Tarihi: | Ara-2001 |
Yayıncı: | Swets Zeitlinger |
Atıf: | Çavuşoğlu, İ. vd. (2002). "Rapid polymerisation with microwave irradiation for transmission electron microscopy". European Journal Of Morphology, 39(5), 313-317. |
Özet: | Successful results of microwave polymerisation of different epoxy formulations have been reported in the literature. The present study was intended to shorten the time needed for polymerisation of epoxy resin by the use of a microwave technique. A standard double fixation and tissue processing was applied to samples of rat kidney tissue. Tissue samples from the control group were polymerised in a conventional oven at 60degreesC for 48h, while tissue from the experimental group was irradiated in a microwave oven, initially at 900 W for 10 min and then at 360 W for another 100 min. During this irradiation, the sealed BEEM capsules were submerged in a water bath, so that the temperature rise was uniform and constant. This resulted in a homogeneous and rapid polymerisation. The cutting properties of the blocks in both groups were similar and no noticeable difference in the quality of the sections was evident when evaluated with TEM. The results showed that the use of a microwave oven reduced the time needed for the polymerisation of Epon blocks without any loss in quality. |
URI: | https://doi.org/10.1076/ejom.39.5.313.7376 http://hdl.handle.net/11452/22629 |
ISSN: | 0924-3860 |
Koleksiyonlarda Görünür: | Scopus Web of Science |
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