Bu öğeden alıntı yapmak, öğeye bağlanmak için bu tanımlayıcıyı kullanınız: http://hdl.handle.net/11452/22629
Başlık: Rapid polymerisation with microwave irradiation for transmission electron microscopy
Yazarlar: Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
0000-0001-5757-8450
Çavuşoğlu, İ.
Minbay, Zehra
Şehime, Temel
ABC-1475-2020
AAG-8385-2021
56009155800
8220935200
6507885442
56636269700
Anahtar kelimeler: Electron microscopy
Epon
Microwave irradiation
Polymerisation
Ultrathin Sections
Preservation
Embedment
Fixatıon
Light
Animalia
Anatomy & morphology
Yayın Tarihi: Ara-2001
Yayıncı: Swets Zeitlinger
Atıf: Çavuşoğlu, İ. vd. (2002). "Rapid polymerisation with microwave irradiation for transmission electron microscopy". European Journal Of Morphology, 39(5), 313-317.
Özet: Successful results of microwave polymerisation of different epoxy formulations have been reported in the literature. The present study was intended to shorten the time needed for polymerisation of epoxy resin by the use of a microwave technique. A standard double fixation and tissue processing was applied to samples of rat kidney tissue. Tissue samples from the control group were polymerised in a conventional oven at 60degreesC for 48h, while tissue from the experimental group was irradiated in a microwave oven, initially at 900 W for 10 min and then at 360 W for another 100 min. During this irradiation, the sealed BEEM capsules were submerged in a water bath, so that the temperature rise was uniform and constant. This resulted in a homogeneous and rapid polymerisation. The cutting properties of the blocks in both groups were similar and no noticeable difference in the quality of the sections was evident when evaluated with TEM. The results showed that the use of a microwave oven reduced the time needed for the polymerisation of Epon blocks without any loss in quality.
URI: https://doi.org/10.1076/ejom.39.5.313.7376
http://hdl.handle.net/11452/22629
ISSN: 0924-3860
Koleksiyonlarda Görünür:Scopus
Web of Science

Bu öğenin dosyaları:
Bu öğeyle ilişkili dosya bulunmamaktadır.


DSpace'deki bütün öğeler, aksi belirtilmedikçe, tüm hakları saklı tutulmak şartıyla telif hakkı ile korunmaktadır.