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Başlık: Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase
Yazarlar: Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.
Kök, Senay
Osman, Bilgen
Kara, Ali
Beşirli, Necati
AAG-6271-2019
ABF-4791-2020
37054138900
15221651200
7102824859
37015388100
Anahtar kelimeler: Polymer science
Adsorption
Eenzymes
Metal-polymer complexes
Afiinity chromatography
Catalase
Adsorption
Purification
Adsorption
Catalysts
Chelation
Chemical analysis
Copolymerization
Copolymers
Enzyme activity
Enzyme immobilization
Ethylene
Ethylene glycol
Monomers
Nitrogen compounds
Scanning electron microscopy
Adsorbed enzyme
Adsorption capacities
Adsorption kinetics
Aspergillus niger
Average diameter
Batch systems
Biocatalytic applications
Copolymer hydrogels
Elemental analysis
Ethylene glycol dimethacrylate
Free enzyme
Glucoamylase
Immobilized enzyme
Initial rate
Kinetic study
Langmuir models
Metal-polymer complexes
Michaelis constants
Monomer units
Optimum temperature
Reversible immobilization
Second-order models
Second-order reaction
SEM
Storage stability
Swelling ratio
Swelling studies
Enzymes
Yayın Tarihi: 5-Haz-2011
Yayıncı: Wiley
Atıf: Kök, S. vd. (2011). "Vinyl triazole carrying metal-chelated beads for the reversible immobilization of glucoamylase". Journal of Applied Polymer Science, 120(5), 2563-2570.
Özet: Poly(ethylene glycol dimethacrylate-1-vinyl-1,2,4-triazole) [poly(EGDMA-VTAZ)] beads with an average diameter of 100-200 μm were obtained by the copolymerization of ethylene glycol dimethacrylate (EGDMA) with 1-vinyl-1,2,4-triazole (VTAZ). The copolymer hydrogel bead composition was determined by elemental analysis and was found to contain 5 EGDMA monomer units for each VTAZ monomer unit. The poly(EGDMA-VTAZ) beads were characterized by swelling studies and scanning electron microscopy (SEM). The specific surface area of the poly(EGDMA-VTAZ) beads was found 65.8 m2/g. Cu 2+ ions were chelated on the poly(EGDMA-VTAZ) beads. The Cu 2+ loading was 82.6 μmol/g of support. Cu2+-chelated poly(EGDMA-VTAZ) beads with a swelling ratio of 84% were used in the immobilization of Aspergillus niger glucoamylase in a batch system. The maximum glucoamylase adsorption capacity of the poly(EGDMA-VTAZ)-Cu2+ beads was 104 mg/g at pH 6.5. The adsorption isotherm of the poly(EGDMA-VTAZ)-Cu 2+ beads fitted well with the Langmuir model. Adsorption kinetics data were tested with pseudo-first- and second-order models. The kinetic studies showed that the adsorption followed a pseudo-second-order reaction model. The Michaelis constant value for the immobilized glucoamylase (1.15 mg/mL) was higher than that for free glucoamylase (1.00 mg/mL). The maximum initial rate of the reaction values were 42.9 U/mg for the free enzyme and 33.3 U/mg for the immobilized enzyme. The optimum temperature for the immobilized preparation of poly(EGDMA-VTAZ)-Cu2+-glucoamylase was 65°C; this was 5°C higher than that of the free enzyme at 60°C. The glucoamylase adsorption capacity and adsorbed enzyme activity slightly decreased after 10 batch successive reactions; this demonstrated the usefulness of the enzyme-loaded beads in biocatalytic applications. The storage stability was found to increase with immobilization.
URI: https://doi.org/10.1002/app.33325
https://onlinelibrary.wiley.com/doi/full/10.1002/app.33325
http://hdl.handle.net/11452/23834
ISSN: 0021-8995
1097-4628
Koleksiyonlarda Görünür:Scopus
Web of Science

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