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Başlık: A valuable adjunct to FNA diagnosis of papillary thyroid carcinoma: In-house PCR assay for BRAF T1799A (V600E)
Yazarlar: Küçükodacı, Zafer
Haholu, Aptullah
Baloğlu, Hüseyin
Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
Akar, Eylem
26634688200
Anahtar kelimeler: Medical laboratory technology
Pathology
BRAF
V600E
Papillary carcinoma
PCR
Thyroid cytology
Mutations
Markers
Cancer
Yayın Tarihi: Haz-2011
Yayıncı: Wiley
Atıf: Küçükodacı, Z. vd. (2011). ''A valuable adjunct to FNA diagnosis of papillary thyroid carcinoma: In-house PCR assay for BRAF T1799A (V600E)". Diagnostic Cytopathology, 39(6), 424-427.
Özet: The diagnostic approach to thyroid nodules generally starts with FNA cytology. However, approximately one-fifth of cytologic evaluations yield indeterminate cytological findings but only 20% of cases with indeterminate thyroid nodule cytology have a cancer diagnosis, emphasizing the need for an effective ancillary test based on FNA material to help prevent unnecessary surgery. Detection of BRAFV600E mutation, the genetic signature of papillary thyroid carcinoma (PTC) in FNA material provides an invaluable diagnostic adjunct to overcome the limitations of FNA cytology. There are many ways to detect V600E, such as direct DNA sequencing, allele-specific PCR and hybridization-based colorimetric methods. In this study, a newer simple PCR method is presented that removes requirements for sequencing special equipment and commercial kits. Two forward primers including the mutant sequence specific (F2), and one common reverse (R) primer were optimized to generate a 241 bp fragment (F1R), an internal PCR control, and a 141 bp fragment (F2R) denoting the presence of V600E. Sensitivity studies revealed that the assay is capable of detecting V600E even in 1 ng of DNA. Direct sequencing data of 241 bp F1R fragment proved the specificity of the assay. For validation studies of the sequence specific multiplex PCR assay, archival FNA slides were used in a group of thyroid lesions including PTC, follicular carcinoma, follicular adenoma, Hashimoto thyroiditis, and benign thyroid nodules. The newer PCR-based method presented in this study is a practical, inexpensive one-step assay to detect the BRAF T1796A mutation on FNA samples. Diagn.
URI: https://doi.org/10.1002/dc.21406
https://onlinelibrary.wiley.com/doi/full/10.1002/dc.21406
http://hdl.handle.net/11452/24103
ISSN: 8755-1039
1097-0339
Koleksiyonlarda Görünür:Scopus
Web of Science

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