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Başlık: Immunohistochemical detection of p53 protein in basal cell skin cancer after microwave-assisted antigen retrieval
Yazarlar: Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Genetik Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
0000-0001-5757-8450
Evke, Elif
Minbay, Zehra
Temel, Şehime
Kahveci, Zeynep
AAG-8385-2021
ABC-1475-2020
8572204200
8220935200
6507885442
6603395784
Anahtar kelimeler: Antigen retrieval
Immunohistochemistry
Microwave irradiation
P53
Skin cancer
Paraffin-embedded tissues
Electron-microscopy
Expression
Tumors
Irradiation
Fixation
Degradation
Carcinoma
Localization
Impregnation
Cell biology
Yayın Tarihi: Şub-2009
Yayıncı: Springer
Atıf: Evke, E. vd. (2009). "Immunohistochemical detection of p53 protein in basal cell skin cancer after microwave-assisted antigen retrieval". Journal of Molecular Histology, 40(1), 13-21.
Özet: p53 is the most frequently altered tumor-suppressor gene in skin cancer. In normal tissues the p53 protein (wild type) has a very short half-life and it is not detectable immunohistochemically. In contrast, the mutant p53 protein has an extended half-life in tumor cells and can be detected by immunohistochemical methods. p53 is widely used as an indicator of tumor aggression and progression. Fixation methods especially formaldehyde based fixation may mask the immunohistochemical detection of p53 protein but antigen retrieval methods enhance the inmmunohistochemical detection of p53 protein by remodification of protein structure. This study was designed to evaluate the efficacy of different fixatives, of microwaving and microwave pretreatment method to retrieve p53 immunoreactivity in paraffin-embedded non-lesioned (adjacent normal tissue) human skin samples or pathological human skin samples diagnosed as basal cell carcinoma. The samples were fixed at RT and/or in microwave oven either in neutral buffered formalin or alcohol for different time periods. For antigen retrieval, the sections were irradiated in a microwave oven for 5 cycles in 10 mM citrate buffer (pH 6.00). In this study the effects of six different fixation methods on the immunohistochemical staining have been investigated in basal cell tumor specimens. The application of antigen retrieval method was also examined and compared. Optimal results were obtained using samples fixed in alcohol either at room temperature (24 h) or in microwave oven.
URI: https://doi.org/10.1007/s10735-008-9208-8
https://link.springer.com/article/10.1007%2Fs10735-008-9208-8
http://hdl.handle.net/11452/24142
ISSN: 1567-2379
Koleksiyonlarda Görünür:Scopus
Web of Science

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