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Başlık: Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts
Yazarlar: Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Klinik Farmakoloji Anabilim Dalı.
Akkoç, Ahmet
Kahraman, Müjdat Müfit
Cansev, Mehmet
Özyiğit, Musa Özgür
M-9071-2019
AAH-2873-2021
AAR-6478-2021
55584229300
6701778876
8872816100
6507338060
Anahtar kelimeler: Agriculture
Zoology
Broiler lung fibroblast
Hypoxia
Hyperosmosis
Matrix metalloproteinases
Pulmonary-hypertension syndrome
Right-ventricular failure
Endothelial-cells
Ascites syndrome
Tissue inhibitors
Drinking-water
Chickens
Sodium
Growth
Hypertrophy
Gallus gallus
Yayın Tarihi: 2011
Yayıncı: Sage Publications
Atıf: Akkoç, A. vd. (2011). ''Effects of hypoxia and hyperosmosis on the expression of matrix metalloproteinases in broiler lung fibroblasts''. Avian Biology Research, 4(1), 6-16.
Özet: This study investigated the effects of hypoxic and hyperosmotic stresses on the matrix metalloproteinases 2 and 9 (MMP-2, MMP-9) and their tissue inhibitors 2 and 1 (TIMP-2, TIMP-1) respectively in cultured lung fibroblasts from broiler chickens. Isolated and sub-cultured primary lung fibroblasts from 1-day-old broilers were exposed to hypoxia and hyperosmosis. The presence of the enzymes and the effects of hypoxia and hyperosmosis on the enzymes in cultured lung fibroblasts, and in the culture media, were evaluated with the streptavidin-biotin-peroxidase method and western blotting using commercially available primary monoclonal antibodies. Short (6 hours) and long term (12 hours) hypoxia, hypoxia + hyperosmosis (6 hours) and hyperosmosis (6 hours) triggered the release of both MMP-2 and TIMP-1 in experimental groups when compared with their controls. Neither MMP-9 nor TIMP-2 antibodies stained the fibroblast cells kept in normoxic, hypoxic or hyperosmotic conditions.
URI: https://doi.org/10.3184/175815511X13000117548916
https://eurekamag.com/research/064/056/064056732.php
http://hdl.handle.net/11452/24302
ISSN: 1758-1559
1758-1567
Koleksiyonlarda Görünür:Scopus
Web of Science

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