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Başlık: Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen
Yazarlar: Ak, Kemal
İleri, İrfan Kamuran
Uludağ Üniversitesi/Veteriner Fakültesi/Dölleme ve Suni Tohumlama Bölümü.
0000-0002-1438-221X
Nur, Zekeriya
Sağırkaya, Hakan
Doǧan, İbrahim
Soylu, Mustafa Kemal
AAH-8821-2021
6508060684
6602400461
36762299000
7003293300
Anahtar kelimeler: Veterinary sciences
Bull
Semen
Acrosome
Membrane functional integrity
Sperm membrane
Bovine spermatozoa
Hypoosmotic test
Ram
Viability
Damage
Yayın Tarihi: Eyl-2005
Yayıncı: Polish Society of Veterinary Sciences
Atıf: Nur, Z. vd. (2005). "Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen". Medycyna Weterynaryjna, 61(9), 991-993.
Özet: The objective of this study was to investigate the effect of low temperature thawing and post-thaw cold shock application on sperm motility, as well as acrosomal and plasma membrane integrities of cryopreserved bull semen. Frozen semen was thawed in a water bath at 5-7 degrees C for 30, 60 or 90 s (low thawed groups), cold shocked (at 5-7 degrees C for 30, 60, 90 s) after thawing at 37 degrees C for 30 s (cold shocked groups), cold for 30 s (control group). The thawing procedure affected the percentages of motile spermatozoa (P < 0.001)and damaged acrosome (P < 0.001) while the bull factor affected the percentages of motile spermatozoa (P < 0.01) and damaged acrosome (P < 00.01). However, swollen tail spermatozoa rates were not affected by the the two factors. There was a significant interaction between thawing procedures and bulls in terms of the studies sperm parameters (P < 0.001). In conclusion, the percentages of motility, intact acrosome and swollen tail spermatozoa of the control group were higher than low-temperature thawed and post-thaw cold-shocked groups. These results indicate that frozen spermatozoa are significantly sensitive to lower temperature not only before but also after thawing.
URI: http://hdl.handle.net/11452/24861
ISSN: 0025-8628
Koleksiyonlarda Görünür:Scopus
Web of Science

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