Bu öğeden alıntı yapmak, öğeye bağlanmak için bu tanımlayıcıyı kullanınız: http://hdl.handle.net/11452/25135
Başlık: Citicoline and postconditioning provides neuroprotection in a rat model of ischemic spinal cord injury
Yazarlar: Uludağ Üniversitesi/Tıp Fakültesi/Beyin ve Sinir Cerrahisi Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.
Türkkan, Alper
Alkan, Tülin
Gören, Bülent
Kocaeli, Hasan
Akar, Eylem
Korfali, Ender
AAH-1792-2021
AAH-1718-2021
25029159600
6601953747
6602543716
6603500567
26634688200
7004641343
Anahtar kelimeler: Apoptosis
Citicoline
Postconditioning
Spinal cord ischemia
Cerebrospinal-fluid drainage
CDP-choline
Reperfusion injury
Cerebral-ischemia
Thoracoabdominal aorta
Focal ischemia
Circulatory arrest
Caspase activation
Aneurysm repair
Brain-damage
Neurosciences & neurology
Surgery
Yayın Tarihi: Haz-2010
Yayıncı: Springer Wien
Atıf: Türkkan, A. vd. (2010). "Citicoline and postconditioning provides neuroprotection in a rat model of ischemic spinal cord injury". Acta Neurochirurgica, 152(6), 1033-1042.
Özet: Ischemic spinal cord injury is a chain of events caused by the reduction and/or cessation of spinal cord blood flow, which results in neuronal degeneration and loss. Ischemic postconditioning is defined as a series of intermittent interruptions of blood flow in the early phase of reperfusion and has been shown to reduce the infarct size in cerebral ischemia. Our study aimed to characterize the relationship between the neuronal injury-decreasing effects of citicoline and ischemic postconditioning, which were proven to be effective against the apoptotic process. Spinal cord ischemia was produced in rats using an intrathoracic approach to implement the synchronous arcus aorta and subclavian artery clipping method. In our study, 42 male Sprague-Dawley rats (309 +/- 27 g) were used. Animals were divided into sham operated, spinal ischemia, citicoline, postconditioning, and postconditioning citicoline groups. Postconditioning was generated by six cycles of 1 min occlusion/5 min reperfusion. A 600 mmol/kg dose of citicoline was given intraperitoneally before ischemia in the citicoline and postconditioning citicoline groups. All rats were sacrificed 96 h after reperfusion. For immunohistochemical analysis, bcl-2, caspase 3, caspase 9, and bax immune staining were performed. Caspase 3, caspase 9, bax, and bcl-2 were used as apoptotic and antiapoptotic markers, respectively. The blood pressure values obtained at the onset of reperfusion were significantly lower than the preischemic values. A difference in immunohistochemical scoring was detected between the caspase 3, caspase 9, bax, and bcl-2 groups. When comparisons between the ischemia (groups 2, 3, 4, and 5) and sham groups (group 1) were performed, a significant increase in caspase 3, caspase 9, bax, and bcl-2 was detected. When comparing the subgroups, the average score of caspase 9 was found to be significantly higher in ischemia group 2. The average score of bcl-2 was also found to be significantly higher in postconditioning and citicoline group 5. It is thus thought that combining citicoline with postconditioning provides protection by inhibiting the caspase pathway and by increasing the antiapoptotic proteins.
URI: https://doi.org/10.1007/s00701-010-0598-5
https://link.springer.com/article/10.1007/s00701-010-0598-5
http://hdl.handle.net/11452/25135
ISSN: 0001-6268
0942-0940
Koleksiyonlarda Görünür:PubMed
Scopus
Web of Science

Bu öğenin dosyaları:
Bu öğeyle ilişkili dosya bulunmamaktadır.


DSpace'deki bütün öğeler, aksi belirtilmedikçe, tüm hakları saklı tutulmak şartıyla telif hakkı ile korunmaktadır.