Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/25820
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dc.date.accessioned2022-04-18T06:21:27Z-
dc.date.available2022-04-18T06:21:27Z-
dc.date.issued2012-05-
dc.identifier.citationAkkoç, A. vd. (2012). "Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors". Biotechnic & Histochemistry, 87(4), 312-319.en_US
dc.identifier.issn1052-0295-
dc.identifier.issn1473-7760-
dc.identifier.urihttps://doi.org/10.3109/10520295.2011.652173-
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.3109/10520295.2011.652173-
dc.identifier.urihttp://hdl.handle.net/11452/25820-
dc.description.abstractWe evaluated the presence of estrogen (ER) and progesterone (PR) receptors, and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes in 18 feline mammary tubulopapillary carcinomas. Immunohistochemistry was performed to localize ER, PR, MMP-2 and MMP-9 in situ. Western blotting and zymographic analyses also were performed to investigate the presence and activities of MMP-2 and MMP-9 enzymes in fresh tissue homogenates. ER immune expression was detected in five samples (27.7%) and PR was positive in sixteen (88.8%) samples. Diffuse cytoplasmic staining of MMP-2 and MMP-9 in neoplastic mammary epithelial cells, stromal fibroblasts and inflammatory cell was evident. MMP-2 and MMP-9 staining was observed also in metastasizing neoplastic cells within lymphatic vessels. MMP-2 and MMP-9 enzymes and their activities in fresh tumor homogenates were demonstrated by zymography. Comparison of MMP-9 gelatinolytic bands from tumor samples and controls revealed a statistically significant difference. We demonstrated elevated MMP-9 and MMP-2 levels in tumor samples by Western blotting; analysis of protein bands revealed 1.9-to-3 fold increase in MMP-9 in tumor samples and the difference was statistically significant. Our results suggest that the expression of MMP-9 can be an important indicator for tumor progression and the possible metastatic nature of feline tubulopapillary carcinomas.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiotechnology & applied microbiologyen_US
dc.subjectCell biologyen_US
dc.subjectEstrogen receptoren_US
dc.subjectFeline mammary tumoren_US
dc.subjectImmunohistochemistryen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectProgesterone receptoren_US
dc.subjectWestern blottingen_US
dc.subjectZymographyen_US
dc.subjectBreast-canceren_US
dc.subjectEstrogen-receptorsen_US
dc.subjectCell-linesen_US
dc.subjectInhibitorsen_US
dc.subjectCarcinomasen_US
dc.subjectCanineen_US
dc.subjectAciden_US
dc.subjectDogsen_US
dc.subjectFelidaeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, westernen_US
dc.subject.meshBreast neoplasmsen_US
dc.subject.meshCat diseasesen_US
dc.subject.meshCatsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene expression regulation, neoplasticen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMammary glands, animalen_US
dc.subject.meshMatrix metalloproteinase 2en_US
dc.subject.meshMatrix metalloproteinase 9en_US
dc.subject.meshReceptors, estrogenen_US
dc.subject.meshStaining and labelingen_US
dc.subject.meshUp-regulationen_US
dc.titleMatrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumorsen_US
dc.typeArticleen_US
dc.identifier.wos000302809100011tr_TR
dc.identifier.scopus2-s2.0-84859790686tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0001-8138-5851tr_TR
dc.identifier.startpage312tr_TR
dc.identifier.endpage319tr_TR
dc.identifier.volume87tr_TR
dc.identifier.issue4tr_TR
dc.relation.journalBiotechnic & Histochemistryen_US
dc.contributor.buuauthorAkkoç, Ahmet-
dc.contributor.buuauthorİnan, Sevda-
dc.contributor.buuauthorSönmez, Gürsel-
dc.contributor.researcheridAAP-7233-2020tr_TR
dc.contributor.researcheridE-3364-2018tr_TR
dc.identifier.pubmed22247959tr_TR
dc.subject.wosBiotechnology & applied microbiologyen_US
dc.subject.wosCell biologyen_US
dc.indexed.wosSCIEen_US
dc.indexed.scopusScopusen_US
dc.indexed.pubmedPubMeden_US
dc.wos.quartileQ4en_US
dc.contributor.scopusid55584229300tr_TR
dc.contributor.scopusid56320836200tr_TR
dc.contributor.scopusid55167435000tr_TR
dc.subject.scopusBreast Tumor; Experimental Mammary Neoplasms; Bitchesen_US
dc.subject.emtreeEstrogen receptoren_US
dc.subject.emtreeGelatinase aen_US
dc.subject.emtreeGelatinase ben_US
dc.subject.emtreeAnimalen_US
dc.subject.emtreeAnimal diseaseen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBlooden_US
dc.subject.emtreeBreast tumoren_US
dc.subject.emtreeCaten_US
dc.subject.emtreeCat diseaseen_US
dc.subject.emtreeEnzymologyen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeGene expression regulationen_US
dc.subject.emtreeImmunohistochemistryen_US
dc.subject.emtreeMetabolismen_US
dc.subject.emtreePathologyen_US
dc.subject.emtreeStainingen_US
dc.subject.emtreeUdderen_US
dc.subject.emtreeUpregulationen_US
dc.subject.emtreeWestern blottingen_US
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