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Başlık: Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors
Yazarlar: Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.
0000-0001-8138-5851
Akkoç, Ahmet
İnan, Sevda
Sönmez, Gürsel
AAP-7233-2020
E-3364-2018
55584229300
56320836200
55167435000
Anahtar kelimeler: Biotechnology & applied microbiology
Cell biology
Estrogen receptor
Feline mammary tumor
Immunohistochemistry
Matrix metalloproteinase
Progesterone receptor
Western blotting
Zymography
Breast-cancer
Estrogen-receptors
Cell-lines
Inhibitors
Carcinomas
Canine
Acid
Dogs
Felidae
Yayın Tarihi: May-2012
Yayıncı: Taylor & Francis
Atıf: Akkoç, A. vd. (2012). "Matrix metalloproteinase (MMP-2 and MMP-9) and steroid receptor expressions in feline mammary tumors". Biotechnic & Histochemistry, 87(4), 312-319.
Özet: We evaluated the presence of estrogen (ER) and progesterone (PR) receptors, and matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes in 18 feline mammary tubulopapillary carcinomas. Immunohistochemistry was performed to localize ER, PR, MMP-2 and MMP-9 in situ. Western blotting and zymographic analyses also were performed to investigate the presence and activities of MMP-2 and MMP-9 enzymes in fresh tissue homogenates. ER immune expression was detected in five samples (27.7%) and PR was positive in sixteen (88.8%) samples. Diffuse cytoplasmic staining of MMP-2 and MMP-9 in neoplastic mammary epithelial cells, stromal fibroblasts and inflammatory cell was evident. MMP-2 and MMP-9 staining was observed also in metastasizing neoplastic cells within lymphatic vessels. MMP-2 and MMP-9 enzymes and their activities in fresh tumor homogenates were demonstrated by zymography. Comparison of MMP-9 gelatinolytic bands from tumor samples and controls revealed a statistically significant difference. We demonstrated elevated MMP-9 and MMP-2 levels in tumor samples by Western blotting; analysis of protein bands revealed 1.9-to-3 fold increase in MMP-9 in tumor samples and the difference was statistically significant. Our results suggest that the expression of MMP-9 can be an important indicator for tumor progression and the possible metastatic nature of feline tubulopapillary carcinomas.
URI: https://doi.org/10.3109/10520295.2011.652173
https://www.tandfonline.com/doi/full/10.3109/10520295.2011.652173
http://hdl.handle.net/11452/25820
ISSN: 1052-0295
1473-7760
Koleksiyonlarda Görünür:PubMed
Scopus
Web of Science

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