Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/27034
Title: The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain
Authors: AlSharari, Shakir D.
Freitas, Kelen
Tracy, Matthew
Damaj, M. Imad
Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi.
Bağdaş, Deniz
15062425700
Keywords: Alpha5
Inflammatory pain
Neuropathic pain
Nicotinic acetylcholine receptors
Cholinergic-receptor
Withdrawal signs
Mice lacking
Spinal-cord
Subunit
Rat
Neurons
Injury
Alpha-5-subunİt
Pharmacology
Pharmacology & pharmacy
Issue Date: 15-Oct-2015
Publisher: Pergamon-Elsevier
Citation: Bağdaş, D. vd. (2015). "The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain". Biochemical Pharmacology, 97(4), Special Issue, 590-600.
Abstract: The aim of the present study was to determine the impact of as nicotinic acetylcholine receptor (nAChR) subunit deletion in the mouse on the development and intensity of nociceptive behavior in various chronic pain models. The role of as-containing nAChRs was explored in mouse models of chronic pain, including peripheral neuropathy (chronic constriction nerve injury, CCI), tonic inflammatory pain (the formalin test) and short and long-term inflammatory pain (complete Freund's adjuvant, CFA and carrageenan tests) in alpha(5) knock-out (1(0) and wild-type (WT) mice. The results showed that paw-licking time was decreased in the formalin test, and the hyperalgesic and allodynic responses to carrageenan and CFA injections were also reduced. In addition, paw edema in formalin-, carrageenan- or CFA-treated mice were attenuated in alpha(5)-K-O mice significantly. Furthermore, tumor necrosis factor-alpha (TNF-alpha) levels of carrageenan-treated paws were lower in alpha(5)-K-O mice. The antinociceptive effects of nicotine and sazetidine-A but not varenicline were alpha(5)-dependent in the formalin test. Both hyperalgesia and allodynia observed in the CCI test were reduced in alpha(5)-K-O mice. Nicotine reversal of mechanical allodynia in the CCI test was mediated through alpha(5)-nAChRs at spinal and peripheral sites. In summary, our results highlight the involvement of the et, nAChR subunit in the development of hyperalgesia, allodynia and inflammation associated with chronic neuropathic and inflammatory pain models. They also suggest the importance of alpha(5)-nAChRs as a target for the treatment of chronic pain.
URI: https://doi.org/10.1016/j.bcp.2015.04.013
https://www.sciencedirect.com/science/article/pii/S000629521500218X
http://hdl.handle.net/11452/27034
ISSN: 0006-2952
Appears in Collections:Scopus
Web of Science

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