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Title: | The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain |
Authors: | AlSharari, Shakir D. Freitas, Kelen Tracy, Matthew Damaj, M. Imad Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi. Bağdaş, Deniz 15062425700 |
Keywords: | Alpha5 Inflammatory pain Neuropathic pain Nicotinic acetylcholine receptors Cholinergic-receptor Withdrawal signs Mice lacking Spinal-cord Subunit Rat Neurons Injury Alpha-5-subunİt Pharmacology Pharmacology & pharmacy |
Issue Date: | 15-Oct-2015 |
Publisher: | Pergamon-Elsevier |
Citation: | Bağdaş, D. vd. (2015). "The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain". Biochemical Pharmacology, 97(4), Special Issue, 590-600. |
Abstract: | The aim of the present study was to determine the impact of as nicotinic acetylcholine receptor (nAChR) subunit deletion in the mouse on the development and intensity of nociceptive behavior in various chronic pain models. The role of as-containing nAChRs was explored in mouse models of chronic pain, including peripheral neuropathy (chronic constriction nerve injury, CCI), tonic inflammatory pain (the formalin test) and short and long-term inflammatory pain (complete Freund's adjuvant, CFA and carrageenan tests) in alpha(5) knock-out (1(0) and wild-type (WT) mice. The results showed that paw-licking time was decreased in the formalin test, and the hyperalgesic and allodynic responses to carrageenan and CFA injections were also reduced. In addition, paw edema in formalin-, carrageenan- or CFA-treated mice were attenuated in alpha(5)-K-O mice significantly. Furthermore, tumor necrosis factor-alpha (TNF-alpha) levels of carrageenan-treated paws were lower in alpha(5)-K-O mice. The antinociceptive effects of nicotine and sazetidine-A but not varenicline were alpha(5)-dependent in the formalin test. Both hyperalgesia and allodynia observed in the CCI test were reduced in alpha(5)-K-O mice. Nicotine reversal of mechanical allodynia in the CCI test was mediated through alpha(5)-nAChRs at spinal and peripheral sites. In summary, our results highlight the involvement of the et, nAChR subunit in the development of hyperalgesia, allodynia and inflammation associated with chronic neuropathic and inflammatory pain models. They also suggest the importance of alpha(5)-nAChRs as a target for the treatment of chronic pain. |
URI: | https://doi.org/10.1016/j.bcp.2015.04.013 https://www.sciencedirect.com/science/article/pii/S000629521500218X http://hdl.handle.net/11452/27034 |
ISSN: | 0006-2952 |
Appears in Collections: | Scopus Web of Science |
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Bağdaş_vd_2015.pdf | 1.12 MB | Adobe PDF | View/Open |
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