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Başlık: The ATP assay, but not the MTT assay, detects further cytotoxicity of the combination of anthracycline-based therapy with histone deacetylase inhibitor (valproic acid) in breast cancer cells
Diğer Başlıklar: ATP testi, MTT testinin aksine, meme kanseri hücrelerinde antrasiklin-bazlı tedavinin histon deasetilaz i̇nhibitörü ile kombinasyonunun yarattıǧı daha i̇leri düzeydeki sitotoksisiteyi tespit edebilmektedir
Yazarlar: İlkay, Elif Armutak
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.
0000-0002-6729-7908
Arı, Ferda
Ulukaya, Engin
K-5792-2018
AAG-7012-2021
24376085300
6602927353
Anahtar kelimeler: Valproic acid
Breast cancer
Apoptosis
FEC protocol
M30 antigen
Caspase-cleaved cytokeratin-18
Luminescence assay
Cemotherapy
Growth
Agent
Drugs
Serum
Biochemistry & molecular biology
Yayın Tarihi: 2010
Yayıncı: Walter De Gruyter
Atıf: Arı, F. vd. (2010). "The ATP assay, but not the MTT assay, detects further cytotoxicity of the combination of anthracycline-based therapy with histone deacetylase inhibitor (valproic acid) in breast cancer cells". Turkish Journal of Biochemistry-Türk Biyokimya Dergisi, 35(4), 293-299.
Özet: Purpose: It has been investigated that whether or not the combination of valproic acid (a histone deacetylase inhibitor) with anthracycline-based chemotherapy (FEC: 5-fluorouracil+epirubicine+ cyclophosphamide) would change the cytotoxic effects of FEC in breast cancer cells. Methods: The effect of valproic acid and its combination with FEC has been tested on MDA-MB-231 and MCF-7 human breast cancer cell lines. Anti-growth effects of treatments were determined by the MTT and ATP assays, while the detection of apoptosis was performed by the caspase-cleaved cytokeratin 18 assay. Results: Valproic acid treatment had anti-growth effect on the cell lines used at clinically achievable dose (0.6 mM). According to the MTT assay, the combination of valproic acid with different doses (50-200% Test Drug Concentration) of FEC did not result in any significant change over FEC-only treatment in both cell lines. However, according to the ATP assay, there has been found that the combination of 100% Test Drug Concentration FEC with valproic acid yielded more efficacy compared to FEC-alone. FEC induced the apoptosis in MCF-7 cells but the addition of valproic acid to FEC did not enhance apoptosis. Conclusion: According to the ATP assay, the use of valproic acid at the clinically achievable dose (0.6 mM) with different doses of FEC further increased the cytotoxic effect of FEC. However, this effect was not observed in the MTT assay. A caution should therefore be taken on the evaluation of the cytotoxic effect of valproic acid in cell lines.
URI: https://web.citius.technology/upload/turkjbiochem/2010/293-299.pdf
http://hdl.handle.net/11452/27381
ISSN: 0250-4685
1303-829X
Koleksiyonlarda Görünür:Scopus
Web of Science

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