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Başlık: IL-6 originated from breast cancer tissue-derived mesenchymal stromal cells may contribute to carcinogenesis
Yazarlar: Sağlam, Özlem
Ünal, Zehra Seda
Subaşı, Cansu
Karaöz, Erdal
Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.
0000-0003-4875-5472
Ulukaya, Engin
K-5792-2018
2-s2.0-84938199098
Anahtar kelimeler: Oncology
IL-6
Tumor microenvironment
Mesenchymal stromal cells
Breast cancer
Stem-cells
Molecular characterization
In-vivo
Growth
Progression
Metasis
Coculture
Yayın Tarihi: Tem-2015
Yayıncı: Sage Publications
Atıf: Sağlam, Ö. vd. (2015). "IL-6 originated from breast cancer tissue-derived mesenchymal stromal cells may contribute to carcinogenesis". Tumor Biology, 36(7), 5667-5677.
Özet: Tumor microenvironment is an important factor, which sustains and promotes the tumors by inflammatory signals. Interleukin-6 (IL-6) is known as a multifunctional cytokine, which is a major activator of the signaling pathway of Janus kinases (JAKs)/signal transducer and activator of transcription 3 (STAT3). In this study, we aimed to investigate the effect of IL-6 in the tumor microenvironment on carcinogenesis. For this purpose, healthy breast tissue-derived stromal cells (HBT-SCs) and malign breast tissue-derived stromal cells (MBT-SCs) were co-cultured with MCF-7 (human breast adenocarcinoma cell line) cells using semipermeable membranes. The cell proliferation was monitored with water-soluble tetrazolium (WST) and carboxyfluorescein succinimidyl ester (CFSE) assays. Protein levels were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot hybridization, while gene expressions were measured by real-time PCR. The results demonstrated that IL-6 protein levels increased significantly in the supernatants of MBT-SCs when they were co-cultured with MCF-7 cells. In accordance with this, the expression of IL-6 was significantly higher in MBT-SCs. Additionally, the expression of STAT3 in MCF-7 cells increased slightly when they were co-cultured with MBT-SCs. Considering together, there is an important interaction between tumor microenvironment and tumor cells mediated by IL-6 signaling. Thereby, the targeting on IL-6 signaling in the treatment of cancer might effectively prevent the tumor progression.
URI: https://doi.org/10.1007/s13277-015-3241-5
https://link.springer.com/article/10.1007/s13277-015-3241-5
http://hdl.handle.net/11452/28257
ISSN: 1010-4283
1423-0380
Koleksiyonlarda Görünür:PubMed
Scopus
Web of Science

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