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Başlık: Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells
Yazarlar: Kumbıçak, Ümit
Kumbıçak, Zübeyde Akan
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
0000-0003-1620-1918
0000-0002-7687-3284
0000-0002-3595-6286
Çavaş, Tolga
Çinkılıç, Nilüfer
Vatan, Özgür
Yılmaz, Dilek
AAH-3508-2021
O-7508-2015
AAH-5296-2021
6602989548
26533892300
16235098100
6701369462
Anahtar kelimeler: Nanotoxicology
Copper-zinc alloy nanoparticles
BEAS-2B cells
Cytotoxicity
Genotoxicity
Metal-oxide nanoparticles
Zno nanoparticles
DNA-damage
Toxicity
Nanomaterials
Release
Nickel
CUO
Food science & technology
Toxicology
Yayın Tarihi: Kas-2014
Yayıncı: Pergamon-Elsevier Science
Atıf: Kumbıçak, Ü. vd. (2014). "Evaluation of in vitro cytotoxicity and genotoxicity of copper-zinc alloy nanoparticles in human lung epithelial cells". Food and Chemical Toxicology, 73, 105-112.
Özet: In the present study, in vitro cytotoxic and genotoxic effect of copper-zinc alloy nanoparticles (Cu-Zn ANPs) on human lung epithelial cells (BEAS-2B) were investigated. XTT test and clonogenic assay were used to determine cytotoxic effects. Cell death mode and intracellular reactive oxygen species formations were analyzed using M30, M65 and ROS Elisa assays. Genotoxic effects were evaluated using micronucleus, comet and gamma-H2AX foci assays. Cu-Zn ANPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Characterization of Cu-Zn ANPs showed an average size of 200 nm and zeta potential of -22 mV. TEM analyses further revealed the intracellular localization of Cu-Zn ANPs in cytoplasm within 24 h. Analysis of micronucleus, comet and gamma-H2AX foci counts showed that exposure to Cu-Zn ANPs significantly induced chromosomal damage as well as single and double stranded DNA damage in BEAS-2B cells. Our results further indicated that exposure to Cu-Zn ANPs significantly induced intracellular ROS formation. Evaluation of M30:M65 ratios suggested that cell death was predominantly due to necrosis.
URI: https://doi.org/10.1016/j.fct.2014.07.040
https://www.sciencedirect.com/science/article/pii/S027869151400372X
http://hdl.handle.net/11452/28490
ISSN: 0278-6915
1873-6351
Koleksiyonlarda Görünür:Scopus
Web of Science

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