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Başlık: Synthesis and crystal structures of novel copper(II) complexes with glycine and substituted phenanthrolines: reactivity towards DNA/BSA and in vitro cytotoxic and antimicrobial evaluation
Yazarlar: Zorlu, Yunus
Yerli, Yusuf
Coşut, Bünyemin
Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Kimya Bölümü.
Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
0000-0002-0483-9642
0000-0002-7687-3284
0000-0002-7528-9529
0000-0002-3595-6286
İnci, Duygu
Aydın, Rahmiye
Vatan, Özgür
Sevgi, Tuba
Yılmaz, Dilek
Demirkan, Elif
Cinkılıç, Nilüfer
G-2201-2019
AAH-8936-2021
O-7508-2015
AAG-7112-2021
ABI-4472-2020
AAH-5296-2021
55082306300
56261495600
16235098100
57191880859
6701369462
23469245200
26533892300
Anahtar kelimeler: Biochemistry & molecular biology
Chemistry, inorganic & nuclear
Antimicrobial activities
Copper(II)
Cytotoxicities
DNA/BSA interactions
Glycine
Single-crystal X-ray diffraction
Substituted phenanthrolines
Dna-binding properties
Oxidative cleavage activity
Human serum-albumin
Schiff-base
Antioxidant activity
Promoted hydrolysis
Phosphate diesters
Antitumor-activity
Ternary complexes
Metal-complexes
Yayın Tarihi: Oca-2017
Yayıncı: Springer
Atıf: İnci, D. vd. (2017). ''Synthesis and crystal structures of novel copper(II) complexes with glycine and substituted phenanthrolines: reactivity towards DNA/BSA and in vitro cytotoxic and antimicrobial evaluation''. Journal of Biological Inorganic Chemistry, 22(1), 61-85.
Özet: New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly)(NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline, and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR, EPR spectroscopy, and cyclic voltammetry. The CT-DNA-binding properties of these complexes have been investigated by thermal denaturation measurements and both absorption and emission spectroscopy. The DNA cleavage activity of these complexes has been studied on supercoiled pUC19 plasmid DNA by gel electrophoresis experiments in the absence and presence of H2O2. Furthermore, the interaction of these complexes with bovine serum albumin (BSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free-energy change (Delta G), enthalpy change (Delta H), and entropy change (Delta S) for BSA + complexes 1 and 2 systems have been calculated by the van't Hoff equation at three different temperatures (293.2, 303.2, and 310.2 K). The distance between the BSA and these complexes has been determined using fluorescence resonance energy transfer (FRET). Conformational changes of BSA have been observed using the synchronous fluorescence technique. In addition, in vitro cytotoxicities of these complexes on tumor cell lines (Caco-2, A549, and MCF-7) and healthy cells (BEAS-2B) have been examined. The antimicrobial activity of the complexes has also been tested on certain bacteria cells. The effect of mono and dimeric in the above complexes is presented and discussed. New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly) (NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR and EPR spectroscopy. They have been tested for their in vitro DNA/BSA interactions by the spectroscopic methods. These complexes exhibited higher cytotoxic and antimicrobial activities. Complex 1 shows better DNA / BSA interactions in comparison to complex 2.
URI: https://doi.org/10.1007/s00775-016-1408-1
https://link.springer.com/article/10.1007/s00775-016-1408-1
http://hdl.handle.net/11452/29315
ISSN: 0949-8257
1432-1327
Koleksiyonlarda Görünür:Scopus
Web of Science

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