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http://hdl.handle.net/11452/29562
Başlık: | Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis |
Yazarlar: | Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı. Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı. Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji ve Reanimasyon Anabilim Dalı. 0000-0001-8111-5958 0000-0001-5428-3630 0000-0003-4820-2288 Dinç, Fatih Akalın, Halis Özakın, Cüneyt Sınırtaş, Melda Kebabçı, Nesrin İşçimen, Remzi Girgin, Nermin Kelebek Kahveci, Ferda AAI-8104-2021 AAG-8392-2021 AAU-8952-2020 AAG-9356-2021 57193412784 57207553671 57200678942 6505818048 56060994000 16645821200 55663009300 6602405968 |
Anahtar kelimeler: | Anesthesiology General & internal medicine Sepsis Real-time polymerase chain reaction Cell culture techniques Polymerase-chain-reaction Stream infections Rapid detection Lightcycler septifast Emergency-department Pathogens Bacterial Assay Identification Procalcitonin |
Yayın Tarihi: | Mar-2016 |
Yayıncı: | Edizioni Minerva Medica |
Atıf: | Dinç, F. vd. (2016). "Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis". Minerva Anestesiologica, 82(3), 301-309. |
Özet: | BACKGROUND: In many cases of suspected sepsis, causative microorganisms cannot be isolated. Multiplex real-time PCR generates results more rapidly than conventional blood culture systems. METHODS: In this study, we evaluated the diagnostic performance of multiplex real-time PCR (LightCycler (R) SeptiFast, Roche, Mannheim, Germany), and compared with blood cultures and cultures from focus of infection in nosocomial sepsis. RESULTS: Seventy-eight nosocomial sepsis episodes in 67 adult patients were included in this study. The rates of microorganism detection by blood culture and PCR were 34.2% and 47.9%, respectively. Sixty-five microorganisms were detected by both methods from 78 sepsis episodes. Nineteen of these microorganisms were detected by both blood culture and PCR analysis from the same sepsis episode. There was statistically moderate concordance between the two methods (kappa=0.445, P<0.001). There was no significant agreement between the blood culture and PCR analysis in terms of microorganism detected (kappa=0.160, P=0.07). Comparison of the results of PCR and cultures from focus of infection revealed no significant agreement (kappa=0.110, P=0.176). However, comparison of the results of PCR and blood cultures plus cultures from focus of infection ( positive blood culture and/or positive culture from focus of infection) showed poor agreement (kappa=0.17, P=0.026). When the blood culture was used as the gold standard, the sensitivity, specificity, positive and negative predictive value of PCR in patients with bacteremia was 80%, 69%, 57% and 87%, respectively. CONCLUSIONS: SeptiFast may be useful when added to blood culture in the diagnosis and management of sepsis. |
URI: | https://europepmc.org/article/med/26022651 http://hdl.handle.net/11452/29562 |
ISSN: | 0375-9393 1827-1596 |
Koleksiyonlarda Görünür: | Scopus Web of Science |
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