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Başlık: Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis
Yazarlar: Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji ve Reanimasyon Anabilim Dalı.
0000-0001-8111-5958
0000-0001-5428-3630
0000-0003-4820-2288
Dinç, Fatih
Akalın, Halis
Özakın, Cüneyt
Sınırtaş, Melda
Kebabçı, Nesrin
İşçimen, Remzi
Girgin, Nermin Kelebek
Kahveci, Ferda
AAI-8104-2021
AAG-8392-2021
AAU-8952-2020
AAG-9356-2021
57193412784
57207553671
57200678942
6505818048
56060994000
16645821200
55663009300
6602405968
Anahtar kelimeler: Anesthesiology
General & internal medicine
Sepsis
Real-time polymerase chain reaction
Cell culture techniques
Polymerase-chain-reaction
Stream infections
Rapid detection
Lightcycler septifast
Emergency-department
Pathogens
Bacterial
Assay
Identification
Procalcitonin
Yayın Tarihi: Mar-2016
Yayıncı: Edizioni Minerva Medica
Atıf: Dinç, F. vd. (2016). "Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis". Minerva Anestesiologica, 82(3), 301-309.
Özet: BACKGROUND: In many cases of suspected sepsis, causative microorganisms cannot be isolated. Multiplex real-time PCR generates results more rapidly than conventional blood culture systems. METHODS: In this study, we evaluated the diagnostic performance of multiplex real-time PCR (LightCycler (R) SeptiFast, Roche, Mannheim, Germany), and compared with blood cultures and cultures from focus of infection in nosocomial sepsis. RESULTS: Seventy-eight nosocomial sepsis episodes in 67 adult patients were included in this study. The rates of microorganism detection by blood culture and PCR were 34.2% and 47.9%, respectively. Sixty-five microorganisms were detected by both methods from 78 sepsis episodes. Nineteen of these microorganisms were detected by both blood culture and PCR analysis from the same sepsis episode. There was statistically moderate concordance between the two methods (kappa=0.445, P<0.001). There was no significant agreement between the blood culture and PCR analysis in terms of microorganism detected (kappa=0.160, P=0.07). Comparison of the results of PCR and cultures from focus of infection revealed no significant agreement (kappa=0.110, P=0.176). However, comparison of the results of PCR and blood cultures plus cultures from focus of infection ( positive blood culture and/or positive culture from focus of infection) showed poor agreement (kappa=0.17, P=0.026). When the blood culture was used as the gold standard, the sensitivity, specificity, positive and negative predictive value of PCR in patients with bacteremia was 80%, 69%, 57% and 87%, respectively. CONCLUSIONS: SeptiFast may be useful when added to blood culture in the diagnosis and management of sepsis.
URI: https://europepmc.org/article/med/26022651
http://hdl.handle.net/11452/29562
ISSN: 0375-9393
1827-1596
Koleksiyonlarda Görünür:Scopus
Web of Science

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