Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/29665
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dc.contributor.authorÇetinkaya, Merih-
dc.contributor.authorÇekmez, Ferhat-
dc.contributor.authorCanpolat, Fuat Emre-
dc.contributor.authorUysal, Sema-
dc.contributor.authorTunç, Turan-
dc.contributor.authorSarıcı, Serdar Ümit-
dc.date.accessioned2022-12-05T11:01:23Z-
dc.date.available2022-12-05T11:01:23Z-
dc.date.issued2013-07-
dc.identifier.citationÇetinkaya, M. vd. (2013). ''CDP-choline reduces severity of intestinal injury in a neonatal rat model of necrotizing enterocolitis''. Journal of Surgical Research, 183(1), 119-128.en_US
dc.identifier.issn0022-4804-
dc.identifier.issn1095-8673-
dc.identifier.urihttps://doi.org/10.1016/j.jss.2012.11.032-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022480412019063-
dc.identifier.urihttp://hdl.handle.net/11452/29665-
dc.description.abstractBackground: Cytidine 5'-diphosphocholine (CDP-choline) is an endogenous intermediate in the biosynthesis of phosphatidylcholine, a contributor to the mucosal defense of the intestine. The aim of this study was to evaluate the possible cytoprotective effect of CDP-choline treatment on intestinal cell damage, membrane phospholipid content, inflammation, and apoptosis in a neonatal rat model of necrotizing enterocolitis (NEC). Methods: We divided a total of 30 newborn pups into three groups: control, NEC, and NEC + CDP-choline. We induced NEC by enteral formula feeding, exposure to hypoxia-hyperoxia, and cold stress. We administered CDP-choline intraperitoneally at 300 mg/kg/d for 3 d starting from the first day of life. We evaluated apoptosis macroscopically and histopathologically in combination with proinflammatory cytokines in the gut samples. Moreover, we determined membrane phospholipid levels as well as activities of xanthine oxidase, superoxide dismutase, glutathione peroxidase, and myeloperoxidase enzymes and the malondialdehyde content of intestinal tissue. Results: Mean clinical sickness score, macroscopic gut assessment score, and intestinal injury score were significantly improved, whereas mean apoptosis score and caspase-3 levels were significantly reduced in pups in the NEC + CDP-choline group compared with the NEC group. Tissue proinflammatory cytokine (interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha) levels as well as tissue malondialdehyde content and myeloperoxidase activities were reduced, whereas glutathione peroxidase and superoxide dismutase activities were preserved in the NEC + CDP-choline group. In addition, NEC damage reduced intestinal tissue membrane phospholipids, whereas CDP-choline significantly enhanced total phospholipid and phosphatidylcholine levels. Long-term follow-up in additional experiments revealed increased body weight, decreased clinical sickness scores, and enhanced survival in CDP-cholineereceiving versus saline-receiving pups with NEC lesions. Conclusions: Our study reports, for the first time, beneficial effects of CDP-choline treatment on intestinal injury in a neonatal rat model of NEC. Our data suggest that CDP-choline may be used as an effective therapeutic agent to prevent NEC.en_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSurgeryen_US
dc.subjectNecrotizing enterocolitisen_US
dc.subjectCDP-cholineen_US
dc.subjectNeonatal raten_US
dc.subjectInflammationen_US
dc.subjectApoptosisen_US
dc.subjectUlcerative-colitisen_US
dc.subjectInflammatory responseen_US
dc.subjectIschemia-reperfusionen_US
dc.subjectMucus barrieren_US
dc.subjectPhosphatidylcholineen_US
dc.subjectCytidineen_US
dc.subjectApoptosisen_US
dc.subjectMechanismsen_US
dc.subjectCiticolineen_US
dc.subjectNecrosisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, newbornen_US
dc.subject.meshApoptosisen_US
dc.subject.meshCytidine diphosphate cholineen_US
dc.subject.meshCytokinesen_US
dc.subject.meshDisease models, animalen_US
dc.subject.meshDrug evaluation, preclinicalen_US
dc.subject.meshEnterocolitis, necrotizingen_US
dc.subject.meshIntestinesen_US
dc.subject.meshNootropic agentsen_US
dc.subject.meshRatsen_US
dc.titleCDP-choline reduces severity of intestinal injury in a neonatal rat model of necrotizing enterocolitisen_US
dc.typeArticleen_US
dc.identifier.wos000320599600023tr_TR
dc.identifier.scopus2-s2.0-84879113583tr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Anatomi Anabilim Dalı.tr_TR
dc.identifier.startpage119tr_TR
dc.identifier.endpage128tr_TR
dc.identifier.volume183tr_TR
dc.identifier.issue1tr_TR
dc.relation.journalJournal of Surgical Researchen_US
dc.contributor.buuauthorCansev, Mehmet-
dc.contributor.buuauthorTayman, Cüneyt-
dc.contributor.buuauthorKafa, İlker Mustafa-
dc.contributor.researcheridM-9071-2019tr_TR
dc.contributor.researcheridAAG-7125-2021tr_TR
dc.relation.collaborationYurt içitr_TR
dc.identifier.pubmed23228325tr_TR
dc.subject.wosSurgeryen_US
dc.indexed.wosSCIEen_US
dc.indexed.scopusScopusen_US
dc.indexed.pubmedPubMeden_US
dc.wos.quartileQ2en_US
dc.contributor.scopusid8872816100tr_TR
dc.contributor.scopusid12243787300tr_TR
dc.contributor.scopusid8450193200tr_TR
dc.subject.scopusNecrotizing Enterocolitis; Prematurity; Newbornen_US
dc.subject.emtreeCaspase 3en_US
dc.subject.emtreeCiticolineen_US
dc.subject.emtreeGlutathione peroxidaseen_US
dc.subject.emtreeInterleukin 1betaen_US
dc.subject.emtreeInterleukin 6en_US
dc.subject.emtreeMalonaldehydeen_US
dc.subject.emtreeMembrane phospholipiden_US
dc.subject.emtreeMyeloperoxidaseen_US
dc.subject.emtreePhosphatidylcholineen_US
dc.subject.emtreePhospholipiden_US
dc.subject.emtreeSodium chlorideen_US
dc.subject.emtreeSuperoxide dismutaseen_US
dc.subject.emtreeXanthine oxidaseen_US
dc.subject.emtreeAnimal experimenten_US
dc.subject.emtreeAnimal modelen_US
dc.subject.emtreeAnimal tissueen_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeCell damageen_US
dc.subject.emtreeCell protectionen_US
dc.subject.emtreeCold stressen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDisease severityen_US
dc.subject.emtreeEnteric feedingen_US
dc.subject.emtreeEnteritisen_US
dc.subject.emtreeEnzyme activityen_US
dc.subject.emtreeFollow upen_US
dc.subject.emtreeHyperoxiaen_US
dc.subject.emtreeHypoxiaen_US
dc.subject.emtreeIntestine injuryen_US
dc.subject.emtreeLipid peroxidationen_US
dc.subject.emtreeNecrotizing enterocolitisen_US
dc.subject.emtreeNewbornen_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreeOxidative stressen_US
dc.subject.emtreePriority journalen_US
dc.subject.emtreeRaten_US
dc.subject.emtreeSurvivalen_US
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