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Başlık: CDP-choline reduces severity of intestinal injury in a neonatal rat model of necrotizing enterocolitis
Yazarlar: Çetinkaya, Merih
Çekmez, Ferhat
Canpolat, Fuat Emre
Uysal, Sema
Tunç, Turan
Sarıcı, Serdar Ümit
Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Anatomi Anabilim Dalı.
Cansev, Mehmet
Tayman, Cüneyt
Kafa, İlker Mustafa
M-9071-2019
AAG-7125-2021
8872816100
12243787300
8450193200
Anahtar kelimeler: Surgery
Necrotizing enterocolitis
CDP-choline
Neonatal rat
Inflammation
Apoptosis
Ulcerative-colitis
Inflammatory response
Ischemia-reperfusion
Mucus barrier
Phosphatidylcholine
Cytidine
Apoptosis
Mechanisms
Citicoline
Necrosis
Yayın Tarihi: Tem-2013
Yayıncı: Academic Press Inc Elsevier Science
Atıf: Çetinkaya, M. vd. (2013). ''CDP-choline reduces severity of intestinal injury in a neonatal rat model of necrotizing enterocolitis''. Journal of Surgical Research, 183(1), 119-128.
Özet: Background: Cytidine 5'-diphosphocholine (CDP-choline) is an endogenous intermediate in the biosynthesis of phosphatidylcholine, a contributor to the mucosal defense of the intestine. The aim of this study was to evaluate the possible cytoprotective effect of CDP-choline treatment on intestinal cell damage, membrane phospholipid content, inflammation, and apoptosis in a neonatal rat model of necrotizing enterocolitis (NEC). Methods: We divided a total of 30 newborn pups into three groups: control, NEC, and NEC + CDP-choline. We induced NEC by enteral formula feeding, exposure to hypoxia-hyperoxia, and cold stress. We administered CDP-choline intraperitoneally at 300 mg/kg/d for 3 d starting from the first day of life. We evaluated apoptosis macroscopically and histopathologically in combination with proinflammatory cytokines in the gut samples. Moreover, we determined membrane phospholipid levels as well as activities of xanthine oxidase, superoxide dismutase, glutathione peroxidase, and myeloperoxidase enzymes and the malondialdehyde content of intestinal tissue. Results: Mean clinical sickness score, macroscopic gut assessment score, and intestinal injury score were significantly improved, whereas mean apoptosis score and caspase-3 levels were significantly reduced in pups in the NEC + CDP-choline group compared with the NEC group. Tissue proinflammatory cytokine (interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha) levels as well as tissue malondialdehyde content and myeloperoxidase activities were reduced, whereas glutathione peroxidase and superoxide dismutase activities were preserved in the NEC + CDP-choline group. In addition, NEC damage reduced intestinal tissue membrane phospholipids, whereas CDP-choline significantly enhanced total phospholipid and phosphatidylcholine levels. Long-term follow-up in additional experiments revealed increased body weight, decreased clinical sickness scores, and enhanced survival in CDP-cholineereceiving versus saline-receiving pups with NEC lesions. Conclusions: Our study reports, for the first time, beneficial effects of CDP-choline treatment on intestinal injury in a neonatal rat model of NEC. Our data suggest that CDP-choline may be used as an effective therapeutic agent to prevent NEC.
URI: https://doi.org/10.1016/j.jss.2012.11.032
https://www.sciencedirect.com/science/article/pii/S0022480412019063
http://hdl.handle.net/11452/29665
ISSN: 0022-4804
1095-8673
Koleksiyonlarda Görünür:PubMed
Scopus
Web of Science

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