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Başlık: Protease immobilization on cellulose monoacetate/chitosan-blended nanofibers
Yazarlar: Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
Uludağ Üniversitesi/Mühendislik Fakültesi/Tekstil Mühendisliği Bölümü.
Demirkan, Elif
Avcı, Tuba
Aykut, Yakup
ABI-4472-2020
23469245200
57200696348
55320835000
Anahtar kelimeler: Materials science
Protease
Enzyme immobilization
Electrospinning
Chitosan
Cellulose acetate
Lipase immobilization
Alpha-amylase
Chitosan
Acetate
Fibers
Nanoparticles
Microspheres
Membranes
Catalysts
Cellulose
Chemical activation
Chemical analysis
Chitin
Chitosan
Differential scanning calorimetry
Electrospinning
Enzyme activity
Enzymes
Fourier transform infrared spectroscopy
Nanofibers
Scanning electron microscopy
Sodium
Sodium hydroxide
Spinning (fibers)
Thermoanalysis
Cellulose acetates
Differential scanning calorimeters
Electrospinning process
Glutaraldehyde activation
Immobilized enzyme activity
Physical adsorption method
Protease
Thermogravimetric analyzers
Enzyme immobilization
Yayın Tarihi: 11-Tem-2017
Yayıncı: Sage Publications
Atıf: Demirkan, E. vd. (2018). ''Protease immobilization on cellulose monoacetate/chitosan-blended nanofibers''. Journal of Industrial Textiles, 47(8), 2092-2111.
Özet: Chitosan-blended cellulose monoacetate nanofibers were prepared through electrospinning process. Neat nanofibers and their sodium hydroxide-treated analogs were used as support surfaces for protease immobilization via physical adsorption method. Morphologies of the nanofibers were observed with a scanning electron microscopy. Chemical analyses were conducted with Fourier transform infrared spectroscopy, and thermal analyses were carried out with differential scanning calorimeter and thermogravimetric analyzer. Immobilized enzyme activities were measured by using casein substrate. In order to test the stability of immobilized enzymes, the tests were repeated until the immobilized enzyme activity was leveled off. The results reveal that well uniform cellulose monoacetate/chitosan nanofibers were obtained, and nanofiber structures are transformed from rounded to more flattened morphology after enzyme activation test. Glutaraldehyde activation has positive effect on sodium hydroxide-treated samples, and the highest immobilization yield as about 83% was observed for glutaraldehyde-treated cellulose monoacetate/chitosan samples. Sodium hydroxide treatment before glutaraldehyde activation shows the best results for protease immobilization on cellulose monoacetate and cellulose monoacetate/chitosan nanofibers. Operational stability increases after sodium hydroxide treatment and glutaraldehyde activation. Glutaraldehyde activation effectively increased the cycle number after sodium hydroxide treatment and about 20% of enzyme activity was still retained after seven cycles at cellulose monoacetate/chitosan samples. This percentage is higher at pure cellulose monoacetate nanofibers than cellulose monoacetate/chitosan nanofibers and measured around 33.5%.
URI: https://doi.org/10.1177/1528083717720205
https://journals.sagepub.com/doi/10.1177/1528083717720205
http://hdl.handle.net/11452/30370
ISSN: 1528-0837
1530-8057
Koleksiyonlarda Görünür:Scopus
Web of Science

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