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Title: | First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey |
Authors: | Göktuna, Pelin Tuncer Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Viroloji Anabilim Dalı. 0000-0002-3411-081X 0000-0003-1793-6879 0000-0003-2468-3945 0000-0001-5969-6127 0000-0001-7676-9033 Aytoğu, Gizem Yeşilbağ, Kadir Toker, Eda Baldan Kadiroğlu, Berfin Ateş, Özer |
Keywords: | BoHV-1 subtyping Molecular characterization Restriction endonuclease analysis Virus isolation |
Issue Date: | 25-Jul-2022 |
Publisher: | Bursa Uludağ Üniversitesi |
Citation: | Aytoğu, G. vd. (2022). ''First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey''. Veteriner Hekimlikte Araştırma Dergisi, 41(2), 80-87. |
Abstract: | Analyzing fingerprints by Restriction Length Polymorphism (RFLP) analysis of complete virus genome which is laborious and time-consuming classifies Bovine Herpervirus-1 (BoHV-1) strains into three subtypes, BoHV-1.1, 1.2a, 1.2b. These subtypes can also being referred according to clinical futures, however, no clear relation was shown. Mostly BoHV-1.2b is associated with genital disease conditions. In this study, BoHV-1 isolate was obtained in a nasal swab sample taken from respiratory tract disease. In this study also, phylogenetic analysis which was targeting UL44 (Glycoprotein C) region of the genome, subtyping was carried out by a recently developed multiplex PCR targeting UL39 and US3 region followed by a RFLP analysis using Hind III enzyme. Also, success of isolation was compared in two continuous cell lines. SFT-R cell line found more susceptible for BoHV-1 field sample isolation than MDBK. The obtained isolate (ID:8640) was serologically undistinguishable from Cooper strain while molecular analysis classified as BoHV-1.2b. Current study reports the first isolation of BoHV-1.2b in Turkey as well as infrequent BoHV-1.2b isolation from clinical case of respiratory illness. Results also highlights the efficiency of PCR based RFLP analysis for easy and quick subtyping but demonstrates the requirement of more investigation to reveal differences based on genetic diversity of BoHV-1 field isolates. |
URI: | https://doi.org/10.30782/jrvm.1082913 https://dergipark.org.tr/tr/download/article-file/2290383 http://hdl.handle.net/11452/31290 |
ISSN: | 1301-3173 |
Appears in Collections: | 2022 Cilt 41 Sayı 2 |
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