1. Açık Erişim@BUU
  2. İndeksli Yayınlar
  3. Web of Science
Please use this identifier to cite or link to this item: http://hdl.handle.net/11452/31464
Title: Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody
Authors: Uludağ Üniversitesi/Veteriner Fakültesi/Hi̇stoloji̇ ve Embri̇yoloji̇ Anabi̇li̇m Dalı.
Erdost, Hatice
AAH-9216-2021
6505787570
Keywords: Anatomy and morphology
Veterinary sciences
Animalia
Gallus gallus
Luteinizing-hormone
Retrieval
Tissue
Pituitary
Sections
Fixation
Cells
Acid
Issue Date: Feb-2004
Publisher: Wiley
Citation: Erdost, H. (2004). “Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody”. Anatomia Histologia Embryologia, 33(1), 49-52.
Abstract: This experiment was conducted to investigate the use of monoclonal mouse anti-human FSH as a primary antibody for the immunohistochemical staining on chicken adenohypophysis paraffin sections. The use of monoclonal mouse anti-human primary antibody on chicken tissues has been developed. The protocol has been modified by comparing the results of the different groups. Briefly, this present protocol suggests modification in two areas; first, the antigen retrieval step, by placing the slides into the buffered citrate in microwave oven at 700 watt for 5 min, 3 times, and antigen-antibody reactions. Secondly, the incubation with the primary antibody (dilution 1/ 100 for anti-FSH) at 37degreesC for 90 min.
URI: https://doi.org/10.1046/j.1439-0264.2003.00511.x
https://onlinelibrary.wiley.com/doi/full/10.1046/j.1439-0264.2003.00511.x
http://hdl.handle.net/11452/31464
ISSN: 0340-2096
Appears in Collections:Web of Science

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