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http://hdl.handle.net/11452/31464
Başlık: | Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody |
Yazarlar: | Uludağ Üniversitesi/Veteriner Fakültesi/Hi̇stoloji̇ ve Embri̇yoloji̇ Anabi̇li̇m Dalı. Erdost, Hatice AAH-9216-2021 6505787570 |
Anahtar kelimeler: | Anatomy and morphology Veterinary sciences Animalia Gallus gallus Luteinizing-hormone Retrieval Tissue Pituitary Sections Fixation Cells Acid |
Yayın Tarihi: | Şub-2004 |
Yayıncı: | Wiley |
Atıf: | Erdost, H. (2004). “Immunohistochemical study on the chicken adenohypophysis using monoclonal mouse anti-human FSH as a primary antibody”. Anatomia Histologia Embryologia, 33(1), 49-52. |
Özet: | This experiment was conducted to investigate the use of monoclonal mouse anti-human FSH as a primary antibody for the immunohistochemical staining on chicken adenohypophysis paraffin sections. The use of monoclonal mouse anti-human primary antibody on chicken tissues has been developed. The protocol has been modified by comparing the results of the different groups. Briefly, this present protocol suggests modification in two areas; first, the antigen retrieval step, by placing the slides into the buffered citrate in microwave oven at 700 watt for 5 min, 3 times, and antigen-antibody reactions. Secondly, the incubation with the primary antibody (dilution 1/ 100 for anti-FSH) at 37degreesC for 90 min. |
URI: | https://doi.org/10.1046/j.1439-0264.2003.00511.x https://onlinelibrary.wiley.com/doi/full/10.1046/j.1439-0264.2003.00511.x http://hdl.handle.net/11452/31464 |
ISSN: | 0340-2096 |
Koleksiyonlarda Görünür: | Web of Science |
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