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Başlık: Screening garlic EST sequences for SSR motifs
Yazarlar: Onus, A. N.
Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.
İpek, Meryem
İpek, Ahmet
AAH-3233-2021
AAK-4655-2021
16031208900
6603912487
Anahtar kelimeler: Biotechnology & applied microbiology
Plant sciences
Agriculture
Allium sativum
Expressed sequence tag
Simple sequence repeats
Genome
Genetic diversity
Markers
Clones
Yayın Tarihi: 2016
Yayıncı: International Society for Horticultural Science
Atıf: İpek, M. ve İpek, A. (2016). "Screening garlic EST sequences for SSR motifs". ed. A. N. Onus. Acta Horticulturae, International Symposium on Biotechnology and Other Omics in Vegetable Science, 1145, 101-104.
Özet: SSR markers are one of the most preferable DNA molecular markers in plant genetic analyses because they are multi allelic, polymorphic, reproducible and transferable among the genetically close species although their development is costly and time consuming. Development of SSR markers can be accomplished with either sequencing of genomic library colonies containing SSR repeats or mining available sequences of related species in gene banks. The second method has been preferred in recent studies due to the dramatic increase in available nucleotide sequences of plant species. For garlic genome, limited SSR markers have been developed so far and development of more SSR markers is needed for detailed genetic characterization and mapping of garlic genotypes. In this study, we screened available garlic EST sequences for SSR motifs in order to generate EST-based SSR markers. We searched repeat motifs containing 2-6 nucleotides. The minimum repeat units criteria was ten for dinucleotides, seven for trinucleotides, five for tetranucleotides, and four for pentanucleotides and hexanucleotides. In total, 86 SSR motifs have been determined using these criteria and the most abounded repeat motifs were determined as (AT/TA)n. Primer pairs designed for some of these SSR motifs were observed as multi allelic and polymorphic among the garlic clones.
Açıklama: Bu çalışma, 29 Nisan-02 Mayıs 2012 tarihlerinde Antalya[Türkiye]’ de düzenlenen International Symposium on Biotechnology and other Omics in Vegetable Science Kongresi‘nde bildiri olarak sunulmuştur.
URI: https://doi.org/10.17660/ActaHortic.2016.1145.16
https://www.actahort.org/books/1145/1145_16.htm
http://hdl.handle.net/11452/33807
ISBN: 978-94-62611-34-4
ISSN: 0567-7572
2406-6168
Koleksiyonlarda Görünür:Scopus
Web of Science

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