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Başlık: Comparison of direct culture versus PCR for the detection of Brucella in aborted fetuses of cattle and sheep in Turkey
Yazarlar: Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.
0000-0003-4337-577X
0000-0002-9212-8743
Büyükcangaz, Esra K.
Şen, Ayşin
Çarli, Kamil Tayfun
Kahya, Serpil
AAL-2323-2020
AAH-1820-2021
AAH-2842-2021
25649139400
7401592869
6601971539
35368679200
Anahtar kelimeler: Veterinary sciences
Real-time PCR
Vaccine strains
Enrichment step
Stomach content
Stomach content
Chain-reaction
Melitensis
Assay
Differentiation
Milk
DNA
Bos
Brucella
Ovis aries
Yayın Tarihi: 23-Nis-2011
Yayıncı: Wiley
Atıf: Büyükcangaz, E. vd. (2011). ''Comparison of direct culture versus PCR for the detection of Brucella in aborted fetuses of cattle and sheep in Turkey". Veterinary Record, 168(16), 430.
Özet: The aim of this study was to detect Brucella in samples from aborted fetuses of sheep and cattle in Turkey using PCR and bacteriological analysis, and to determine the sensitivity and specificity of the PCR. Organ homogenates from 38 aborted fetuses of cattle and 56 aborted fetuses of sheep were tested. All organ homogenates were cultured for bacteriological analysis, and all of the homogenates and the Brucella isolates obtained by culture were examined with a commercial PCR kit. On bacteriological analysis, Brucella species were found in 30 (31.9 per cent) of the 94 organ homogenates, eight (21.1 per cent) of which were from cattle and 22 (39.3 per cent) from sheep. Using PCR, a total of 29 (30.9 per cent) homogenates were positive for Brucella species, eight (21.1 per cent) of which were from cattle and 21 (37.5 per cent) from sheep. Compared with the bacteriological method, the diagnostic sensitivity and specificity of the PCR kit used in this study were 83 per cent and 94 per cent, respectively.
URI: https://doi.org/10.1136/vr.c7003
https://bvajournals.onlinelibrary.wiley.com/doi/full/10.1136/vr.c7003
http://hdl.handle.net/11452/23862
ISSN: 0042-4900
Koleksiyonlarda Görünür:Scopus
Web of Science

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