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Başlık: Effect of freezing rate on acrosome and chromatin integrity in ram semen
Yazarlar: Uludağ Üniversitesi/Veterinerlik Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.
Uludağ Üniversitesi/Veterinerlik Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
0000-0003-1976-1814
Nur, Zekariya
Zık, Berrin
Üstüner, Burcu
Tütüncü, Şerife
Saǧirkaya, Hakan
Özgüden, Cansel G.
Günay, Ülgen
Doǧan, İbrahim
AAH-2635-2021
AAG-7238-2021
AAH-8821-2021
R-8366-2018
AAH-9810-2021
6508060684
6507763192
18937724600
16551094700
6602400461
16550925100
55901087200
36762299000
Anahtar kelimeler: Veterinary sciences
Apoptosis
Freezing rate
Ram semen
Cryoprotective agents
Membrane integrity
Spermatozoa frozen
Bull sperm
Fertility
Extender
Glycerol
Cryopreservation
Insemination
Osmolality
Yayın Tarihi: 2011
Yayıncı: Ankara Üniversitesi
Atıf: Nur, Z. vd. (2011). "Effect of freezing rate on acrosome and chromatin integrity in ram semen". Ankara Üniversitesi Veteriner Fakültesi Dergisi, 58(4), 267-272.
Özet: The objective of the present study was to investigate the effect of different freezing rates on post-thaw sperm motility, acrosome defect, and sperm chromatin structure and apoptotic activity in ram semen. Collected semen was diluted at 1:5 (semen/extender) with Bioxel (R) (IMV technologies France) at 30 degrees C and then cooled to 5 degrees C within 1h. Cooled semen was subjected to the equilibration for 2 hours. Equilibrated semen was frozen in 0.25 ml straw at two different cooling rates (slow: 0.5 degrees C/min from 5 to -20 degrees C and fast: 5 degrees C/min from 5 to -20 degrees C). Both groups were frozen from -20 to -120 degrees C at 25 degrees C/min and stored in liquid nitrogen until use. Post-thaw (37 degrees C/30 min) sperm motility, defected acrosome (Pisum sativum agglutinin fluorescein conjugate, FITC PSA), sperm chromatin structure determined by Acridin Orange (AO) and apoptotic activity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) were evaluated. Post-thaw sperm motility, acrosome defect, AO and TUNEL for slow frozen semen were 42.8 +/- 8.8%, 31.6 +/- 12.9%, 2.9 +/- 2.4% and 2.8 +/- 1.6%, and for fast frozen semen were 36.5 +/- 9.9%, 24.7 +/- 11.1%, 3.3 +/- 2.2% and 6.3 +/- 3.4%, respectively. Post-thaw semen analyses showed that there was no significant difference between two freezing curves in terms of acrosome defect, sperm chromatin damage (AO). However, a significant difference was found for post-thaw semen motility between two groups (P<0.05). In conclusion, while the slow freezing procedure improved post-thaw sperm motility, acrosome and chromatin integrities and apoptotic index in ram spermatozoa did not show any significant difference between freezing rates.
URI: https://doi.org/10.1501/Vetfak_000000248
http://vetjournal.ankara.edu.tr/tr/pub/issue/44729/555881
http://hdl.handle.net/11452/23936
ISSN: 1300-0861
1308-2817
Koleksiyonlarda Görünür:Scopus
TrDizin
Web of Science

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