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Başlık: Effect of storage period of red blood cell suspensions on helper T-cell subpopulations
Yazarlar: Güvenç, Furkan
Uludağ Üniversitesi/Tıp Fakültesi/Dr. Raşit Durusoy Kan Bankası.
Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.
Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Mikrobiyoloji/İmmünoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.
Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.
0000-0001-7625-9148
0000-0003-0463-6818
Bal, Salih Haldun
Kumaş, Levent Tufan
Budak, Ferah
Göral, Güher
Oral, Haluk Barbaros
Heper, Yasemin
HJY-9001-2023
F-4657-2014
AAH-6506-2021
K-7285-2012
56191003300
26665534000
6701913697
6603453166
7004498001
57191480128
Anahtar kelimeler: Hematology
TRIM
Immunomodulation
Transfusion
Th cell subgroups
Leucoreduction
Randomized controlled-trial
Transfusion-related immunomodulation
Colorectal-cancer surgery
Independent risk-factor
Growth-factor-beta
Acute lung injury
Cardiac-surgery
Whole-blood
Postoperative infection
Leukocyte depletion
Yayın Tarihi: 2018
Yayıncı: Simtipro Srl
Atıf: Bal, S. H. vd. (2018). ''Effect of storage period of red blood cell suspensions on helper T-cell subpopulations''. Blood Transfusion, 16(3), 262-272.
Özet: Background. The aim of this study was to investigate the immunological alterations that occur during the storage of erythrocyte suspensions which may lead to transfusion-related immunomodulation following allogeneic blood transfusion. Materials and methods. One part of the erythrocyte suspensions obtained from donors was leucoreduced while the other part was not. The leucoreduced (LR) and non-leucoreduced (NL) erythrocyte suspensions were then further divided into three equal amounts which were stored for 0, 21 or 42 days prior to measurements, by enzyme-linked immunosorbent assays, of cytokine levels in their supernatants. T-helper (Th) lymphocyte subgroups and gene expression were analysed in the NL erythrocyte suspensions by flow cytometry and real-time polymerase chain reaction, respectively. Results were compared to those of storage day 0. Results. By day 21, the number of Th2 cells had increased significantly and the numbers of Th1, Th22 and Treg cells had decreased significantly in the NL erythrocyte suspensions. On day 42 the numbers of Th2 and Treg cells in the NL suspensions were significantly increased while the number of Th1 cells was significantly decreased. The levels of transcription factors (TBX21, GATA3, and SPI.1) were significantly decreased on days 21 and 42, and AHR, FOXP3 and RORC2 levels were significantly increased on day 42 in NL erythrocyte suspensions. The decrease in interleukin-22 and increase in transforming growth factor-beta levels found in NL erythrocyte suspensions on day 21 were statistically significant. Elevated levels of interleukin-17A were found in both LR and NL erythrocyte suspensions on day 42. Discussion. Our results suggest that allogeneic leucocytes and cytokines may play significant roles in the development of transfusion-related immunomodulation.
URI: https://doi.org/10.2450/2017.0238-16
https://www.bloodtransfusion.it/bt/article/view/323
http://hdl.handle.net/11452/34939
ISSN: 1723-2007
Koleksiyonlarda Görünür:Scopus
Web of Science

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